水稻条纹病毒 (RSV) 引起的水稻条纹叶枯病是我国水稻生产中重要的病害之一，RSV编码的沉默抑制子p2是一个核质穿梭蛋白。p2通过与核仁和柯浩体 (cajal bodies, CBs) 互作来影响RSV的系统侵染，CBs是动态结构，常能协助蛋白完成出入细胞核的运动；同时，p2还能与水稻沉默抑制子SGS3互作，SGS3是RDR6介导的RNAi抗病途径的一个重要组分，两者在细胞质中聚合成siRNA bodies。本项目拟在此基础上，通过酵母双杂交、共定位和双荧光互补 (BiFC) 研究p2与细胞＂小体＂即CBs和siRNA bodies的互作，并结合病毒诱导基因沉默 (VIGS) 技术、western blot等方法，研究这些互作在RSV侵染中的作用。所获结果将揭示p2在病毒复制和运动中的关键作用及其过程，为深入解析p2的生物学功能提供科学依据。

Rice stripe disease, caused by Rice stripe virus (RSV), is one of economically important diseases in rice, p2，a RSV-encoded silencing suppressor, is a nucleoplasm shuttle protein. p2 plays a role in virus systemic infection through interacting with nucleolus and cajal bodies (CBs), CBs is dynamic structures, and always aid proteins to enter or exit the nucleus. Meanwhile, p2 interacts with OsSGS3, which is a significant factor of RDR6-mediated antiviral RNAi pathway, SGS3 binds RDR6 to small cytoplasmic bodies, designated siRNA bodies. On this basis, yeast two hybrid, co-localization and BiFC methods will be used to study the relationship between p2 and cell ＂bodies＂, CBs and siRNA bodies. Moreover, the roles of interactions during RSV infection will be dissected employing virus-induced gene silencing (VIGS) technique, western blot method and so on. The results will reveal the critical roles of p2 during viral replication, and provide scientific basis for understanding the biological function of p2.