水稻条纹病毒 (RSV) 引起的水稻条纹叶枯病是我国水稻生产中重要的病害之一，RSV编码的沉默抑制子NS2是一个核质穿梭蛋白，能与寄主本氏烟纤维蛋白 (NbFib2) 互作。本项目拟利用RSV可以侵染本氏烟的优点并结合病毒诱导的基因沉默 (VIGS) 技术，研究NS2与NbFib2互作在RSV病毒复制和扩散中的作用；通过亚细胞定位观察、双荧光互补 (BiFC)、免疫共沉淀 (CO-IP) 等方法弄清NS2作为穿梭蛋白是否协同RSV的运动蛋白NSvc4参与病毒的胞间运动。所获结果将揭示NS2在病毒复制和运动中的关键作用及其过程，为深入解析NS2的生物学功能提供科学依据。

Rice stripe disease, caused by Rice stripe virus (RSV), is one of economically important diseases in rice. NS2, a RSV-encoded RNA silencing suppressor, is a nucleoplasm shuttle protein and interacts with Nicotiana benthamiana Fibrillarin2 (NbFib2). Because N. benthamiana can be infected by RSV, this project will use N. benthamiana as host to dissect the roles of interaction between NS2 and NbFib2 in RSV replication and transmission by employing virus-induced gene silencing (VIGS) technique. In addition, subcellular localization observation, BiFC and CO-IP methods will be used to determine whether NS2 and NScv4, a movement protein of RSV, cooperatively involve in cell-to-cell movement. The results will reveal the critical roles of NS2 during viral replication, and provide scientific basis for understanding the biological function of NS2.