乳腺癌已成为最常见的恶性肿瘤之一，进展转移仍是乳腺癌患者死亡的主要原因。代谢异常是肿瘤细胞区别于正常细胞的重要标志，固醇调节元件结合蛋白-1（SREBP1）作为脂质代谢途径中的重要转录因子，可将致癌信号转化为肿瘤脂质代谢信号，但是目前我们对SREBP1在肿瘤进展转移中所起的作用却知之甚少。前期通过乳腺癌临床样本，我们发现SREBP1的表达与乳腺癌进展转移显著相关，提示SREBP1是乳腺癌的潜在致癌因子。为了更深入研究SREBP1在乳腺癌进展转移中的重要作用，本项目拟利用稳定干扰SREBP1的乳腺癌细胞系进行体内外实验，分析SREBP1对乳腺癌细胞增殖凋亡、浸润转移及脂质代谢的影响，并利用基因芯片技术探讨其具体分子机制，并通过检测SREBP1在良恶性乳腺组织中的表达以评价其临床应用价值。此项课题的完成，将有助于阐明SREBP1在乳腺癌进展转移中所起的作用，也将为乳腺癌的诊治提供新的靶点。

Breast cancer has become one of the most common malignant tumors, progression and metastasis were the main causes for the death of breast cancer patients. Abnormal metabolism is one of the hallmarks for tumor cells. Sterol regulatory element-binding protein 1 (SREBP1), an important transcription factor in lipogenesis pathway, connect oncogenic signal with lipogenesis signal for tumor cells. However, little is known about the role of SREBP1 in breast cancer progression and metastasis. Previously, we have found that the expression of SREBP1 in breast cancer tissue samples was significantly correlated with the progression and prognosis of breast cancer, suggesting that SREBP1 was the potential oncogenic factor. To further investigate the role of SREBP1 in breast cancer progression and metastasis, we used the stable breast cancer cell lines which has been knockdown of SREBP1 to clarify the effect of SREBP1 on breast cancer proliferation, apoptosis, invasion, metastasis and lipogenesis both in vitro and in vivo. We will further apply gene microarray to identify the detailed molecular mechanism. In order to evaluate the clinical significance of SREBP1, SREBP1 expression in the normal and malignant breast tissues will also be detected and analyzed. Our work will help elucidate the role of SREBP1 in the progression and metastasis of breast cancer, and also help identify novel targets for the diagnosis and treatment for breast cancer.