基于系统生物学探讨功能性消化不良脾虚证的生物学基础

目前对中医脾虚证的研究存在以下问题：（1）研究以脾虚证动物模型为研究对象，研究结果具有局限性，难以全面真实反映脾虚证的科学内涵；（2）采用传统的研究方法从某一角度进行研究难以揭示脾虚证的全貌。而以整体性研究为特征、阐释生命整体性和动态性的系统生物学方法为深入诠释脾虚证理论科学内涵提供了可能。功能性消化不良的临床表现和核心病机与脾虚证契合，在基因组上存在相应的特征基因群，在代谢组上表现为菌群与宿主共代谢的特征代谢物，经典健脾药具有系统调控作用。本课题拟从脾虚证与肠道菌群密切相关入手，肠道微生态及其与宿主共代谢的组学变化可能是脾虚证科学内涵的重要基础，也是健脾中药治疗脾虚证的重要靶标；通过分析临床组学研究的“大数据”，找到特异性代谢物指标，筛选和验证药物作用靶点，是揭示脾虚证理论科学内涵的重要路径，也将为临床功能性胃肠病脾虚证的诊断和疗效评价指标的客观化、规范化研究奠定基础。

Current studies on spleen deficiency syndrome of TCM have the following problems: (1) research on the animal model of spleen deficiency as the research object. The results of the study have limitations, it is difficult to truly and fully reflect the scientific connotation of spleen deficiency syndrome; (2) Using traditional research methods from a certain point of view can not reveal the whole picture of spleen deficiency syndrome.However,The systematic biological approach to the characteristics of the whole study, the interpretation of the whole life and the dynamic characteristics of the system provides a possibility for the deep interpretation of the scientific connotation of the theory of spleen deficiency syndrome.Clinical manifestation and core pathogenesis of functional dyspepsia consistent with spleen deficiency syndrome.There is a corresponding characteristic gene group in the genome.The metabolism group showed the characteristic metabolites of bacteria and host,and classic spleen medicine has the function of system regulation.This subject is to start with the spleen deficiency syndrome and intestinal flora closely related.The changes of intestinal micro ecology and its host metabolism may be an important basis for scientific connotation of spleen deficiency syndrome, and also an important target for spleen deficiency syndrome .Through the analysis of the "big data" in the study of clinical group, to find the specific metabolites, screening and validation of drug targets,which is an important way to reveal the scientific connotation of the theory of spleen deficiency syndrome, and it will lay the foundation for the diagnosis and evaluation of the clinical function of the spleen and stomach deficiency syndrome and the evaluation index of clinical efficacy.