复合ad-SVF的柔性石墨烯微阵列凝胶促进膀胱老化介导UAB逼尿肌修复的作用及机制

膀胱老化介导的UAB目前尚无理想的治疗方法，逼尿肌、微血管内皮损伤及炎症微环境改变是UAB发生的重要原因。既往研究发现，ad-SVF为一种重要的干细胞群，在组织损伤修复中具重要作用，但移植效率低下为目前有待解决的难题。我们前期研究发现，自体ad-SVF可促进逼尿肌及血管内皮修复，改善逼尿肌功能。柔性石墨烯微阵列凝胶可提高ad-SVF的移植效率，促进COX-2、mPGES-1、PGE2等分泌，在UAB修复中可能发挥着重要作用。我们推测，复合ad-SVF的柔性石墨烯微阵列凝胶可增强ad-SVF修复能力，炎症微环境及COX-2/mPGES-1/PGE2/EP通路发挥着关键性作用。本项目拟制备复合ad-SVF的柔性石墨烯微阵列凝胶，采用膀胱芯片、细胞示踪、microPET-CT等，从结构和功能两方面探讨其对逼尿肌、微血管内皮修复以及炎症微环境的影响及机制，可为膀胱老化介导UAB的治疗开辟新途径。

Currently, there is no ideal treatment for UAB mediated by bladder aging. The damage of detrusor, microvascular endothelium and the change of inflammatory microenvironment are the important causes of UAB. Previous studies have found that ad-SVF is an important group of stem cells, which plays an important role in injury repair, but the low efficiency of transplantation is a difficult problem to be solved. Our previous study found that autologous ad-SVF can promote detrusor and vascular endothelial repair, improve detrusor function. The flexible graphene microarray gel may improve the transplant efficiency of ad-SVF. The flexible graphene microarray gel combined with ad-SVF can promote the secretion of COX-2, mPGES-1 and PGE2, and the repair of UAB. We speculate that flexible graphene microarray gel with ad-SVF can enhance the repair capability of ad-SVF, while inflammatory microenvironment and COX-2/mPGES-1/PGE2/EP pathway molecules play a key role. In this project, flexible graphene microarray gel with ad-SVF was prepared. Cell tracer and micro PET-CT were used to study the structure and function of flexible graphene microarray gel in vivo and in vitro, and to explore its effect on detrusor, microvascular endothelial repair and inflammatory microenvironment, which can open up a new way for the treatment of bladder aging-mediated UAB.