课题基金基金详情
DNA甲基化译码蛋白CsMBD13a在茶树休眠解除中的作用机制
结题报告
批准号:
31972461
项目类别:
面上项目
资助金额:
57.0 万元
负责人:
郝心愿
学科分类:
茶学
结题年份:
2023
批准年份:
2019
项目状态:
已结题
项目参与者:
郝心愿
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中文摘要
茶树是以幼嫩叶片为利用对象的多年生常绿木本作物。春季萌芽早晚影响茶叶的经济价值和茶树对气温波动的应对能力,一直是茶树品种选育过程中备受关注的重要生物学性状之一。项目组前期研究表明茶树越冬芽休眠解除的早晚是影响春茶发芽期的重要因素,DNA甲基化译码蛋白CsMBD13a介导的主动去甲基化过程在茶树休眠解除调控中发挥核心作用。为深入解析CsMBD13a的作用机制,本项目将在基因序列及表达分析的基础上,利用转基因等技术手段明确基因的功能及启动子响应模式;通过上游转录因子和互作蛋白的全面鉴定揭示CsMBD13a的分子调控机制;开展转基因植株gDNA甲基化水平检测,转录组、全基因组甲基化测序分析,结合休眠解除阶段茶树越冬芽的CHIP-Seq分析发掘CsMBD13a的识别区域和下游调控基因,阐明其在茶树休眠解除中的作用机制。为茶树休眠机理的全面揭示、不同萌发物候茶树品种选育和分子设计育种提供理论支撑。
英文摘要
Tea plant is a perennial evergreen woody species that use fresh leaves as their utilization object. The date of bud break in spring affects the economic value of tea products and the ability of young shoots in coping with temperature fluctuations, which has always been one of the most important biological characteristics in the process of tea variety breeding. Previous studies showed that the dormancy release of tea overwintering buds was an important factor affecting the bud break of tea plant in spring. It was hypothesized that active demethylation mediated by DNA methylation decoding protein CsMBD13a plays a central role in the regulation of dormancy release of tea plants. In order to further understand the role and mechanism of CsMBD13a, this project will perform gene sequence and expression analysis initially, and then use the transgenic technique to clarify gene function and promoter response mode. Moreover, comprehensive identification of upstream transcription factors and interaction proteins will be carried out to reveal the molecular regulation mechanism of CsMBD13a. Furthermore, gDNA methylation level detection, transcriptome analysis, genome-wide methylation sequencing analysis of transgenic plants, combing with CHIP-Seq analysis of overwintering buds of tea plants during dormancy release period, will be performed to identify the recognition regions and downstream regulatory genes of CsMBD13a. Finally, the role and mechanism of CsMBD13a in dormancy release regulation of tea plants will be clarified to improve understanding of bud dormancy and laid a theoretical foundation for the selection and molecular design breeding of tea varieties with different sprouting phenology.
期刊论文列表
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专利列表
DOI:10.48130/bpr-2021-0010
发表时间:2021
期刊:Beverage Plant Research
影响因子:--
作者:Yao Chen;Yuteng Li;Hengze Ren;Jianrong Zhou;Lu Wang;Yajun Yang;Xinyuan Hao;Xinchao Wang
通讯作者:Xinchao Wang
DOI:10.3389/fpls.2023.1263606
发表时间:2023
期刊:Frontiers in plant science
影响因子:5.6
作者:
通讯作者:
DOI:10.16420/j.issn.0513-353x.2021-1218
发表时间:2023
期刊:园艺学报
影响因子:--
作者:李宇腾;陈瑶;任恒泽;李聪聪;王浩乾;曹红利;岳川;郝心愿;王新超
通讯作者:王新超
DOI:--
发表时间:2021
期刊:茶叶科学
影响因子:--
作者:陈瑶;张伟富;任恒泽;熊飞;张豪杰;姚利娜;刘莹;王璐;王新超;杨亚军;郝心愿
通讯作者:郝心愿
DOI:--
发表时间:2023
期刊:茶叶科学
影响因子:--
作者:李聪聪;王浩乾;叶玙璠;陈瑶;任恒泽;李宇腾;郝心愿;王新超;曹红利;岳川
通讯作者:岳川
茶树CsZHD9和CsCBF1协同调控越冬芽休眠及萌发的分子机制
CsDAM基因在茶树越冬芽休眠调控中的作用机制
国内基金
海外基金