滨海湿地是连接海陆环境、微生物多样性高度复杂的特殊生态系统。湿地微生物可通过底物代谢、电子传递等方式相互耦合，协同驱动碳氮硫等元素的生物地球化学循环。然而，微生物在不同时空尺度下如何耦合并驱动元素循环的机制尚未清楚。本项目基于前期所积累的不同时空条件下大量的滨海湿地宏基因组和宏转录组数据及相应的环境参数，提出采用多组学数据驱动的研究思路，从微生物组及其转录活性的角度，利用本团队开发的创新性方法tmap，将多组学数据和环境参数相结合，以“微生物协同作用形成活性功能模块，共同驱动碳氮硫元素循环”为科学假设，建立滨海湿地的“物种–功能–元素”微生物组拓扑学分析框架，从微生物组活性功能模块的角度研究滨海湿地碳氮硫元素循环的耦合机制。研究成果有助于为深入理解水圈微生物碳氮硫循环的耦合机制提供创新性视角，有效推动数据驱动的水圈微生物组研究模式。

Coastal wetlands are unique ecosystems that connect land and sea with highly complex microbial diversity. Wetland microorganisms can be coupled with each other through substrate metabolism, electron transfer, etc., and cooperatively drive the biogeochemical cycles of carbon, nitrogen and sulfur. However, the mechanism of how microorganisms couple and drive element circulation at different spatiotemporal scales is not yet clear. Based on our large amount of metagenomic and metatranscriptomic data and corresponding environmental parameters of coastal wetlands under different spatiotemporal conditions, we propose a multi-omics data-driven research roadmap. From the perspective of the microbiome and its transcriptional activity, we are applying the innovative method tmap developed by the team to combine multi-omics data and environmental parameters, with the "microorganism synergistically forming active functional modules to jointly drive the carbon, nitrogen and sulfur element cycles" as the scientific hypothesis. It is to establish a "species-function-element" microbiome topological analysis framework for coastal wetland researches, and to study the coupling mechanisms of carbon, nitrogen and sulfur element cycles in coastal wetlands from the perspective of microbiome active functional modules (AFM). The research results will be helpful to provide an innovative perspective for the in-depth understanding of the coupling mechanism of hydrosphere microbial carbon, nitrogen and sulfur cycles, and effectively promote the data-driven research model of hydrosphere microbiome.