负载SDF-1的牙龈素响应型材料促进原位牙周组织再生的研究

牙周炎是由牙周致病菌侵染造成的局部炎症反应，严重时可导致牙齿脱落，且与多种系统性疾病密切相关。在牙周炎临床治疗中，传统的牙周组织工程存在长期抗菌效果不佳，外源性干细胞干性丧失，支架材料可塑性差等问题，达不到理想的牙周再生效果。本项目在前期研究中开发了“牙龈素响应型抗菌材料”，同时对基质细胞衍生因子-1（stromal cell-derived factor-1，SDF-1）的原位促再生作用进行了深入探索的基础上，拟通过牙龈素响应型抗菌材料负载SDF-1，合成具有靶向抗菌作用和原位促再生功能的复合材料，通过体外实验探究其抗菌效果、生物相容性、募集干细胞能力等，通过体内实验探究其长期靶向抗菌和促进原位牙周组织再生的协同作用效果和机制。该复合材料可适用于不同形状牙周缺损，有望通过长效抗菌和招募干细胞归巢，为临床牙周病治疗提供可以靶向抗菌和原位促再生的双功能材料，从而提升牙周再生性治疗的效果。

Periodontitis is a local inflammatory response caused by the infection of periodontal pathogens, which can cause tooth loss in severe cases, and is closely related to a variety of systemic diseases. In the clinical treatment of periodontitis, traditional periodontal tissue engineering has several drawbacks such as inefficient long-term antibacterial effect, loss of exogenous stem cell stemness, and poor plasticity of the scaffold, which cannot achieve the ideal periodontal regeneration effect. In the previous research, we developed a “gingivalin-responsive thermosensitive scaffold material” and deeply explored the in situ regeneration mechanism of stromal cell-derived factor-1 (SDF-1). This project intends to synthesize a thermosensitive composite material with targeted antibacterial effect and in situ regeneration promotive function by loading SDF-1 with a gingivalin-responsive thermosensitive scaffold material. Using in vitro experiments, we intend to explore its antibacterial effect, biocompatibility and stem cell recruitment ability. Through in vivo experiments, we plan to investigate its long-term targeted antibacterial and synergistic effects on periodontal tissue regeneration in situ and the underneath mechanism. The thermosensitive composite material can adapt to periodontal defects of different shapes. Based on the long-term in situ antibacterial and recruitment of stem cells, our project aims to provide a bifunctional materials with targeted antibacterial effect and in situ regeneration potential for the treatment of periodontitis, and upgrade periodontal tissue regeneration quality eventually.