促分裂原活化蛋白激酶途径（ MAPK cascade）是真核生物中保守的信号转导系统，在调节植物响应水分胁迫中发挥重要作用。条斑紫菜是潮间带大型海藻的典型代表，具有高效的水分胁迫适应机制。目前条斑紫菜MAPK级联途径应答水分胁迫机制的研究报道有限，项目组研究发现，条斑紫菜TDY型PyMAPK2对失水胁迫响应灵敏，但在进化上独立于高等植物TDY型MAPK, 暗示其拥有特殊调控机制。. 本项目以PyMAPK2为出发点，系统揭示其在转录、翻译和酶活性水平对水分胁迫的响应模式，通过蛋白互作技术筛选PyMAPK2激活的下游靶因子，阐释PyMAPK2所在的级联途径应答水分胁迫的调控机制。以期深入理解条斑紫菜对水分胁迫的适应机制，拓展对植物适应干旱胁迫机制的认识，为条斑紫菜抗逆育种提供重要支持。

The MAPKs cascades, as the conservative signal transduction system among the eukaryotes, play the important roles in responding to water stress. Pyropia yezoensis is the representative of seaweeds inhabiting intertidal zone. The researches in our group showed, PyMAPK2 is very sensitive to desiccation. Based on the research limitation and the current progresses in our group, in this research, we will focus on those MAPKs genes including PyMAPK2 responding to water stress at transcript and translation levels, and comprehensively reveal the response patterns under water stress. By the protein interactome technology, the identification of the target protein of PyMAPK2 and the regulators will elucidate the regulation networks of P. yezoensis adaption to water stress. Our researches will be benefit to understand P. yesoensis adaption mechanisms to water stress and extend the cognition of plant tolerance against the drought, which also provide the supports for the drought tolerance breeding of P. yezoensis .