肌动蛋白微丝对环境因子的适应机制成为胁迫生物学领域的研究热点。周期性潮起潮落引起的水分胁迫，是海洋潮间带环境的主要极端因子之一。条斑紫菜是潮间带大型海藻的典型代表，具备高效的水分胁迫适应机制。项目组研究发现，条斑紫菜肌动蛋白ACT2参与了促分裂原活化蛋白激酶MAPK2介导的级联途径响应水分胁迫，且微丝结构肌动蛋白结合蛋白ADF1对水分胁迫反应灵敏，暗示条斑紫菜的微丝是其适应潮间带水分胁迫的重要机制之一。. 本申请以潮间带大型海藻条斑紫菜为研究对象，在细胞水平揭示水分胁迫下微丝的细胞动力学变化，阐明ACT2和ADF1在转录和翻译水平对水分胁迫的响应模式，通过蛋白互作技术建立ACT2和ADF1响应水分胁迫的调控模型，系统阐释微丝响应水分胁迫的动力学及调控机制。以期深入阐明潮间带大型海藻对水分胁迫的适应机制，拓展对微丝功能的认识，从而进一步理解潮间带物种群落分布的理论基础。

The mechanism about F-actin filament adaption to abiotic factors has been the research hot in the field of stress biology. The water stress resulting from daily changes of tides in intertidal zone, is the most severe factor. Pyropia yezoensis is the representative of seaweeds inhabiting intertidal zone and can tolerate dissication-rehydration stress effectively. We found that PyMAPK2 was involved in the cascade pathway mediated by PyMAPK2, and actin-binding factor ADF1 was sensitive to water stress. This implied that F-actin filament played important roles in adaption to water stress of interdal zone.. In this research, we will observe the cytoskeleton dynamics of F-actin filament under desiccation-rehydration, and identify the response patterns of ACT2 and ADF1 under water stress. By the protein interactome technology, we will construct the regulatory models of ACT2 and ADF1 responding to water stress at after transcriptional and translational level. Our researches will be benefit to understand P. yesoensis adaption mechanisms to water stress and extend the cognition of F-actin filament tolerance against the drought, which will also provide the supports for understanding species distribution of intertidal zone.