芳樟醇是中药金银花的有效成分，我们前期研究发现，芳樟醇具有显著的抗巴氏杆菌活性，通过课题组建立的信号传导通路研究平台发现芳樟醇抗炎作用显著，但在感染性疾病中其抗炎作用机制尚不明确。因此针对巴氏杆菌感染时LPS导致剧烈的炎症反应而使疾病恶化，本项目拟应用分子生物学和现代免疫学相关技术，从Nrf2信号传导通路入手，评价芳樟醇在Nrf2野生型、基因缺失型小鼠中构建的多杀性巴氏杆菌感染和LPS诱导的败血症模型中的保护作用；并采用Western免疫印迹、Real-time PCR、EMSA、免疫荧光等方法，检测芳樟醇对Nrf2-Keap1及Nrf2相关的NF-κB和MAPKS等信号传导通路的影响，确定调控炎症反应的关键节点，寻找芳樟醇作用的Pathway和新的药物靶标，深入阐明芳樟醇抗炎的分子机制，为芳樟醇作为先导化合物进一步开发应用研究及新药靶标的筛选奠定基础。

Linalool is an effective component of traditional Chinese medicine honeysuckle. In our previous research, it has been shown that linalool has significant antibacterial activity against Pasteurella and anti-inflammatory role by using our signal transduction pathway platform. But its anti-inflammatory mechanism of action in the bacterial infections is not clear. Aiming at this problem of LPS induced intense inflammatory response that goes against the rehabilitation of the disease with Pasteurella induced infection, in this project, with the molecular biological and modern immunological related techniques, starting from Nrf2 signaling pathways, the protective effect and regulatory impact analysis in Nrf2-Keap1, Nrf2-associated signal transduction pathway such as NF-κB and MAPKS of linalool to the pathological models with Pasteurella multocida induced infection and LPS-induced sepsis will be attempted to detect using Nrf2 knockout(Nrf2(-/-)) and Nrf2 wild-type(+/+) mice; Nrf2-Keap1, Nrf2-associated signal transduction pathway such as NF-κB and MAPKS will be analyzed in vitro with Western Blot, Real-time PCR, electrophoretic mobility shift assay(EMSA), and cyto-immunofluorescence staining technique for determining key knots in the regulating the inflammatory response, searching for the pathway and some new drug targets by the action of linalool, attempting to further elucidate the molecular mechanism of linalool's anti-inflammatory properties. The research consequences may offer theoretical guidance and experimental reference for the treatment of the inflammation with Pasteurella induced infection. It may lay the foundation for the further exploitation and application of the linalool as a lead compound and for the new drug targets screening.