长童期性状是指大豆品种在短日照条件下延迟营养生长期，该性状的应用打破了大豆不能在低纬度地区种植的限制，改变了大豆生产和贸易格局。申请人前期结果表明，J（控制长童期性状的关键基因）结合到大豆开花期核心调控因子E1启动子的LUX结合元件负调控E1基因转录表达，进而影响生育期和产量，暗示J与LUX形成蛋白复合物且LUX与E1基因启动子直接结合。但是，大豆中LUX基因功能及分子机制的研究尚属空白。申请者拟通过基因敲除和过表达遗传转化获得转基因株系，鉴定LUX基因对光周期的影响；利用酵母杂交系统、蛋白体外结合试验和染色体免疫共沉淀等技术解析LUX蛋白与J蛋白的相互作用并验证LUX对E1基因的调控作用；利用近等基因系或转基因株系验证LUX基因与已克隆生育期基因之间的调控关系。LUX基因功能的研究和作用机理的解析，不仅能够促进对光周期调控大豆开花机制的深入认知，而且可以为分子育种提供理论依据和育种材料。

The long-juvenile (LJ) trait in soybean [Glycine Max (L.) Merr.] has been defined as delayed flowering time under short-day conditions. This characteristic is especially important as it increases the range of adaptation of soybean in low latitudes and improves the yield for low latitudes. In our previous study, we found that, the major gene for long-juvenile trait, J depends genetically on the legume-specific flowering repressor, E1, and J protein physically associates with the E1 promoter of LBS site (conserved DNA binding sites for LUX) to down-regulate its transcription, but the mechanism about LUX has not been elucidated. In this research, we planned to get transgenic plant, which will help us to identified the influence of LUX on photoperiod and yield. In addition, we demonstrated the complex LUX-J and LUX binds to E1 promoter by yeast system, Pull-down and ChIP-PCR. At last the genetically regulative relationship of J with known maturity genes will be determined using these maturity gene isolines and transgenic lines. This study not only made us to understand more deeply of the molecular mechanism for LUX and the photoperiod, but also provided theory basis and genetic resources for molecular breeding, which eventually improved the soybean yield.