本研究拟运用液相自组装法，以各种形貌和结构的纳米钛酸盐为初级结构，系统研究纳米颗粒间的组装机制，优化相关制备条件，获得形貌及结构可控的TiO2介观晶体。研制空间分辨的多通道光电化学传感系统，凝练纳米材料的光电化学活性评估方法，高通量筛选在可见光区具有高分辨光电响应的TiO2介观晶体，探索其显微结构与光电化学活性之间的关系。以碳纳米角为构筑基元制备探针识别界面，多功能化的TiO2介观晶体为光电化学探针，利用空间分辨多通道传感界面的信号可区分性，结合信号放大策略，构建竞争型免疫传感器，实现对多种真菌毒素的高灵敏同时测定，以满足真菌毒素的现实检测需求。借助于介观晶体和碳纳米角的独特结构及其形成的协同作用，该型传感器在光电化学传感领域具有极高的通用性和实用性。本研究将推动真菌毒素的多组分高通量快速检测、新型介观晶体材料的传感应用，并拓展光电化学传感的应用范围，具有重要的社会价值和科学意义。

The aim of this proposal is to explore the synthesis of TiO2 mesocrystals by self-assembly of titanate nanometer material in solution-phase. The systematic analysis of synthesis conditions and synthesis strategies will be made to illustrate the formation mechanism of TiO2 mesocrystals. On the basis of the above results, the controlled synthesis of TiO2 mesocrystals will be realized. Through developing the multi-channel photoelectrochemical biosensor and establishing the evaluation procedure of screening the suitable photoelectrochemical nanometer materials for biosensing application, the high resolution and sensitive TiO2 mesocrystals are chose as the photoelectrochemical probes. This exploration will elucidate the relationship between microstructure of TiO2 mesocrystals and its corresponding photoelectrochemical action, and establish the theoretical foundation for the photoelectrochemical biosensor based on TiO2 mesocrystals. By employing carbon nanohorns to fabricate the sensing interface, a competitive immunosensor is designed for ultrasensitive multiplexed measurement of various mycotoxins using photoelectrochemical signal of TiO2 mesocrystals. This attempt will meet the requirement of mycotoxin determination in practice. Due to the unique properties of carbon nanohorns and TiO2 mesocrystals, this photoelectrochemical biosensor owns versatility and practical applicability. The relative result will provide a promising ultrasensitive simultaneous multiplexed immunoassay approach for mycotoxin determination. Furthermore, it will expand the application scope of TiO2 mesocrystals and photoelectrochemical sensing.