本研究拟设计、集成外泌体膜蛋白LSR靶向识别单元和电致化学发光示踪单元，获得具有上皮性卵巢癌源性外泌体膜蛋白靶向识别能力的温致增敏型多功能探针。基于功能化碳纳米角组装体构建癌源性外泌体的捕获电极。通过“类三明治”识别模式，捕获电极依次识别并结合癌源性外泌体和纳米金多功能探针。借助光热效应，通过光源调制，获得反映外泌体膜蛋白LSR表达情况的增敏型电致化学发光信号。进一步调制光源信号，致使该多功能探针温度持续升高，诱导固载的外泌体裂解。在捕获电极附近设计两个传感界面，结合信号放大传感策略、电致化学发光信号编码技术和空间分辨电致化学发光阵列的信号可区分性，实现对外泌体胞浆内CA125和HE4的定量检测。结合样本分析，探讨上皮性卵巢癌外泌体所介导的LSR、CA125和HE4等标志物作为联合检测指标，早期诊断卵巢癌的可行性及其临床意义。这将推动外泌体介导的早期诊断方法的研究。

This study is designed to integrate membrane protein LSR targeted identification units and electrochemiluminescent tracer units to obtain multifunctional probe, whose response could be enhanced by alternating temperature. The capture electrode for carcinogenic exosomes is constructed based on functional carbon nanohorns assembly. By means of the sensing mode of the sandwich type, the captured electrode is combined with the carcinogenic exosomes and multifunctional probes. Through the photothermal effect and light source modulation, the sensitized electrochemiluminescent signal is obtained which reflects the expression of the outer secretion membrane protein of LSR. To further alternating light source, the temperature of the multifunction probe continues to rise, and the disruption of membranes of exsomes is induced. Two sensing interfaces are designed near the capture electrode. Combined signal amplification sensing strategy, electrochemiluminescence signal coding technology and signal differentiability of spatial resolution electrochemiluminescence array, the quantitative detection of CA125 and HE4 in exosomes is realized. In combination with the sample analysis, the feasibility and clinical significance based on LSR, CA125 and HE4 of exsomes as the biomarkers of ovarian cancer are discussed. This will promote the study of the early diagnosis method based on exosome and provide a new method for early diagnosis and treatment of ovarian cancer.