饲喂频率可影响动物的生长性能和养分利用，但其内在机制尚不完全清楚。本申请项目以猪为研究对象，旨在从肠道微生物角度解析饲喂频率影响猪肠道养分利用的机制。首先通过饲养试验研究不同饲喂频率下猪的生长性能等表型指标；再利用肠道瘘管猪探讨不同饲喂频率对肠道微生物生物钟和机体肠道生物钟的影响，利用微生物组、代谢组、宏转录组等多组学技术研究肠道微生物及其代谢对宿主不同采食模式的响应规律；分析不同组织生物钟相关基因表达，探究宿主肠道生物钟和微生物生物钟的关联；最后，通过肠道菌群干预，研究抑制肠道菌群后饲喂频率如何影响肠道生物钟和养分利用，揭示饲喂频率影响猪肠道养分利用的微生物学机制。该研究可有助于我们进一步认识肠道微生物代谢与机体整体代谢互作机制，同时也为高效优质猪生产的营养调控策略提供理论指导。

The feeding frequency can affect the growth performance and nutrient utilization of animals, but the mechanism is not completely clear. This project aims to investigate the mechanism of effects of feeding frequency on gut nutrient utilization of pigs from the angle of intestinal microbiota. First, animal trials will be conducted to study the effects of different frequency of feeding on the phenotypic growth performance of pigs. Then, pigs filled with intestinal fistula will be used to investigate the effects of different feeding frequency on intestinal microflora and intestinal circadian clock. Mutil-omis techniques will be applied to study the responses of intestinal microbiota on the different patterns of feeding. Expression of biological clock related genes in different tissue will be determined to analysis the correlation between intestinal biological clock and microbial biological clock. Finally, we will investigate the effects of feeding frequency on intestinal biological clock and nutrient utilization after the intervention on gut microbiota, and reveal the microbial mechanism of effects of feeding frequency on pig intestinal nutrient utilization. This project can help us further understand the interaction mechanism of intestinal microbial metabolism and overall metabolism of the body, and also provides theoretical guidance for nutritional control strategies of pig production in high quality.