热应激会导致畜禽发生非特异性心脏损伤（细胞骨架破坏、细胞增殖停滞、细胞凋亡以及细胞内外钙离子稳态失衡）而发生猝死。小热休克蛋白αB-Crystallin是主要定位于细胞骨架中的分子伴侣，与上述损伤所处的通路密不可分。本项目通过真核表达及RNA干扰，分别建立稳定过表达和抑制表达αB-Crystallin的心肌细胞系，并从细胞损伤、细胞修复、细胞内外钙离子稳态、细胞凋亡通路等方面对αB-Crystallin保护心肌细胞的功能进行比较研究；用免疫共沉淀方法，研究αB-Crystallin 与Hsp27及desmin之间的蛋白互作机制；用染色质共沉淀的方法，揭示HSFs与αB-Crystallin之间的调控关系。通过对αB-Crystallin保护心肌细胞功能及其分子机制的全面研究，为解决因热应激导致的机体损伤及生产损失提供理论支持。

Heat stress could induce non-specific myocardium damage even sudden death in livestock. Several factors could induce injury of animal heart. Such as the cytockeleton of cardium was destroyed, inhibition of cell proliferation, cell apoptosis, disruption of calcium homeostasis etc. Small heat shock protein αB-Crystallin acts as moleculer chaperone may involve in above mentioned pathological process to protecting myocardium when subjected to heat shock. In this study, gene clone and RNA interference will be firstly used for selecting stable over expression and low expression αB-Crystallin myocardial cell lines. Then several detection indices（cytoskeleton, cell recovery, calcium homeostasis, apoptosis cascades etc.）will be detected by using Flow cytometer and confocal microscope et al. According to above comparative study, the mechanism of αB-Crystallin in heat stressed myocardium will be investigated. Besides, the interaction between αB-Crystallin and Hsp27 will be detected by co-immunoprecipitation. Further, whether regulation factors HSFs could regulate αB-Crystallin gene will be tested by using Chromatin Immunoprecipitation(CHIP). After synthesizing all the data based on above experiments, the protective role and regulation of αB-Crystallin will be well and truly investigated. The results will lead the clinical pharmacy when livestock being subjected to heat stress.