乙烯是影响月季花朵开放的关键因素，而乙烯受体是结合乙烯分子、开启乙烯信号转导途径的首要环节。申请者的前期工作已经证明Rh-ETR3是月季花瓣生长中控制乙烯响应的主要受体蛋白。通过分裂泛素化酵母双杂交筛选及测序，目前已获得Rh-ETR3候选互作蛋白39个。在此基础上，本项目拟对筛选到的候选互作蛋白进行互作验证，分离其中确实结合并影响Rh-ETR3信号输出的互作蛋白，并对其进行功能鉴定，确定其与Rh-ETR3的互作方式。并探索其在乙烯调节的月季花瓣生长过程中，对乙烯受体Rh-ETR3信号输出的调节模式，进而较为系统地分析月季花瓣内Rh-ETR3蛋白在花瓣生长过程中及响应乙烯过程中的转录后调节机制。项目的实施将为解析乙烯对月季花朵开放的调节机制提供新的试验证据和理论基础。实践上，有望为月季采后性状遗传改良提供基因资源，并可为月季切花新型高效保鲜剂的开发提供依据。

Ethylene plays crucial role in flower opening driven by petal-expansion. Ethylene receptor facilites the function of binding ethylene molecule and initiate signal transduction pathway.Our previous works demonstrated that Rh-ETR3 is the predominent receptor for mediating ethylene during petal development in rose.Using the split-ubiquitin membrane yeast two-hybrid system, we screened 39 interating protein cadidates of Rh-ETR3. In present work, we try to confirm the interation between cadidate proteins and Rh-ETR3. For convinced interacting proteins, we try to characterize their function by overexpression in Arabidopsis and silencing in rose petals. According to fluorescence co-localization and immuno-copercipitation analysis, we will figure out the interation pattern of Rh-ETR3 and its interacting proteins, and the regulatory mechanism of interacting protein upon ethylene signaling. This work will provide novel evidents to understand underlying post-transcriptional regulation mechanism of Rh-ETR3 in petal development of rose flower. And this work might be contributed to develop and improve post-handling techniques