月季是全球第一大切花，花瓣张开形成花型是月季观赏品质的形成过程,直接决定了切花品质和经济价值。采后流通中，乙烯会加速月季花瓣张开，引起花型畸变，导致严重损耗。然而，乙烯影响花瓣运动的作用机制目前尚不清楚。申请者发现，乙烯通过影响月季花瓣基部不对称生长，调节了花瓣运动和花朵开放。综合组学筛选和基因功能分析，发现转录因子RhPMP1可响应乙烯、参与调节花瓣运动。本项目拟以RhPMP1为切入点，系统分析其介导乙烯信号的上游途径及下游基因网络。拟通过原位杂交检测RhPMP1时空表达特性；通过在月季中稳定过表达和基于CRISPR的基因编辑，确认RhPMP1在花瓣运动中的功能；通过Y1H筛库鉴定其上游调节子；通过ChIP-seq和RNA-seq鉴定RhPMP1的直接靶基因。综合上述，本项目将有望初步阐明乙烯调节花瓣运动、影响花朵开放品质的分子机制，以期为月季品种改良和采后品质维持技术研发提供理论基础。

Rose (Rosa sp.) is the most important ornamental crop worldwide and thus is economically very important. During postharvest handling, ethylene could cause severe deterioration of flower opening quality by accelerating petal movement while inhibiting petal expansion. So far, however, the underlying mechanism of ethylene in flower opening remains largely unknown. Previously, we found that petal movement was driven by asymmetric growth of petal base, and ethylene could enhance this process. Combined RNA-seq and qRT-PCR, we isolated dozens of ethylene-responsive transcription regulator genes from petal base. According to VIGS-based functional analysis, we found that silencing a homeodomain TF gene resulted in defect in ethylene-accelerated petal movement. Therefore, this gene was named as RhPMP1 (Petal movement-related protein 1). Here, we attempt to understand how RhPMP1 plays its role in ethylene-enhanced petal movement. We will isolate the promoter of RhPMP1 and screen the possible regulator upstream to RhPMP1 gene by using Yeast One Hybrid Screening. We will generate rose transgenic lines overexpressing RhPMP1 and harboring edited RhPMP1 locus to confirm its function, respectively. And then, we will identify the targeting loci of RhPMP1 protein by combining ChIP-seq and RNA-seq approaches using RhPMP1-GFP-OE lines. This work will establish the possible working pathway of RhPMP1 in ethylene-regulated petal movement. Moreover, this work will be beneficial for molecular breeding of rose plants and improvement of postharvest handling of rose flowers in the future.