隐孢真菌一般可分为块菌和类块菌，主要是指子实体生长在地下或部分露出地表，子实层封闭在子实体内，孢子依靠动物进行传播的类群。多数隐孢真菌为外生菌根真菌，是森林中连接树木与动物的重要环节。相比基于传统分类的多样性分析，高通量测序分析具有全面、高效、经济等优点。本研究拟采用系统分类与metabarcoding相结合的方式，分析云南松林下隐孢真菌的物种多样性与时空分布，结合传播动物的群落组成，探讨云南隐孢真菌与小型哺乳动物之间可能存在的协同演化关系。该研究对云南松林生物多样性保护与资源持续利用具有指导意义，是我国隐孢真菌研究中具有前瞻意义的一次尝试。

Sequestrate fungi, which include truffles and truffle-like fungi, are characterized by the hypogeous or partly emergent basidiomes that enclose their hymenophoral trama and most of these fungi have spores that are dispersed by animals.Most of sequestrate fungi are ectomycorrhizal fungi, and serve as critical biological links that connect trees and animals. Compared with taxonomy based diversity analysis, high-throughput sequencing as a tool make the diversity analysis comprehensive and cost-effective. The analysis of sequestrate fungi diversity and spatiotemporal distribution in Pinus yunnanensis forest will be carried out by the combination of traditional and metabarcoding identification. The putative co-evolution between sequestrate fungi and small mammals in Yunnan, will be explored. This work has a guiding significance for the conservation of biodiversity and their sustainable utilization in Pinus yunnanensis forest, and will be a prospective research on sequestrate fungi in China.