生物矿化细胞对珍珠层形成的作用及其调控机制

珍珠的形成是典型的生物矿化过程，具有十分重要的研究价值，外套膜和珍珠囊是珍珠质形成的器官，它们的生理活动伴随着珍珠质的分泌形成珍珠的过程，决定着形成珍珠的品质，珍珠形成机理的研究一直是人们所关注的重点。本课题以合浦珠母贝为研究材料，从外套膜和珍珠囊中分离具有持续分裂能力的细胞，评价其在体外生长分裂能力，探索其体外分泌基质蛋白和调控珍珠层形成的机理；筛选出促矿化细胞增殖的外源刺激物，用于指导培养基优化，以期获得适合贝类细胞增殖生长的培养基；建立原代外套膜和珍珠囊培养细胞矿化研究模型，用于研究矿化细胞分泌基质蛋白的调控机制，初步筛选出对贝壳生长和珍珠质形成起到重要调控作用的矿化细胞种类，并对其在珍珠质生物矿化中的调控作用和调控机理进行深入研究，揭示矿化细胞在珍珠质形成中的功能和作用机理，提出珍珠生产的生物学措施。

The formation of the pearl is a typical biomineralization process, has a very important studying value. the mantle and pearl sac are the organs of the formation of of nacre, and their life activities along with the secretion of nacre form a pearl, decided to the quality of the pearl. The mechanism of the formation of the pearl has been the focus of attention by the people. We will use the pearl oyster Pinctada fucata, we isolated cells with continued division capacity from the mantle and pearl sac, to evaluate the growth and division activities in vitro, to explore the reason of the secretion of matrix proteins and the formation of the nacre in vitro; the use of cell biology analysis the exogenous irritants of proliferating cells in vivo, used to guide the optimization of culture medium, in order to obtain suitable medium; we established mineralization model of primary mantle and pearl sac cell culture for studying the regulation mechanism of the mineralized cells to secrete matrix proteins, initial screening the cell types play an important regulatory role in the formation of shell and nacre, and its regulatory role and regulatory mechanism of nacre biomineralization will reveal the mechanism of the mineralization cells in the formation of nacre ,we will get biological measures for the pearls production.