产后抑郁在我国产后妇女的发病率为15-20%，严重时有杀害婴儿倾向。对产妇的身心健康，及婴儿的发育危害严重。临床应用的抗抑郁药物，起效慢、副作用大及影响哺乳等，致众多家庭放弃药物治疗。神经肽在疾病治疗方面具有起效快、副作用小的特点。甘丙肽（GAL）广泛存在于与情绪相关的脑区，通过三个G蛋白偶联受体发挥不同的生物学功能。在束缚应激、急性强迫游泳等抑郁模型中的研究表明，GalR1/3发挥着致抑郁样作用，而GalR2发挥的是抗抑郁样作用。但在产后抑郁模型中GAL是否可通过GALS参与到产后抑郁的发病机制中还未见探索。我们课题组在卵巢雌激素撤退及孕期束缚两种产后抑郁动物模型上，利用可精准定位脑核团的激光显微切割捕获技术，定位注射慢病毒包装的GALR1-SiRNA等方法，研究GAL及GALRs表达变化，验证GAL是否可通过GALR1在产后抑郁模型中发挥抗抑郁作用，为产后抑郁的治疗提供新的生物学靶点。

The incidence of postpartum depression(PPD) in postpartum women was 15-20 % , in severe cases, PPD can be accompanied to harm the infant, and cause serious harm to the baby's development and mother’s health. Currently antidepressant drugs in clinical applications had some abuse,for exampe, the slow onset , side effects, and other effects of breast-feeding , causing many patients and their families nto give up drugs. The characteristics of neuropeptied were work fast and small side effects. Galanin (GAL) is widely present in brain areas associated with emotion , through three G protein-coupled receptors GalR1, GalR2, GalR3 play different biological functions. In the restraint stress , and other acute forced swimming depression model studies show , GalR1 / 3 plays induced depression -like effects in animals , but GalR2 play is antidepressant-like effects . However, the mechanism of the model of postpartum depression of GALRs and GAL has not been reported . So we first duplicated two postpartum depression animal models :ovarian estrogen withdraw and restraint stress during pregnancy PPD animal models, then,we use laser capture microdissection (LMD) method , injecting GALR1-SiRNA into special brain region. Study the expression of GAL and GALRs between control and PPD model groups. ,indentify Knockdown of GalR1 in the special region of PPD rats modeal produce antidepressant-like effects, and provide new biological targets for the treatment of postpartum depression .