人类皮肤定居着复杂而多样的微生物，皮肤共生菌参与形成生物屏障和细胞代谢，具有免疫调节功能。目前，已通过高通量筛选获得对特应性皮炎(AD)金黄色葡萄球菌(S.aureus)抑制活性的凝固酶阴性葡萄球菌(CoNS)数株，CoNS对AD具有防治作用，但具体机制知之甚少。本项目利用AD小鼠模型，拟通过CoNS-S.aureus体外共培养，采用RNA-seq，HPLC和Biolog分析CoNS通过怎样的代谢途径和分子机制抑制S.aureus的生长；皮肤共生菌移植(AMT)AD小鼠皮炎创面，宏基因组和宏转录组分析微生物菌群结构和功能，检测宿主细胞的基因表达调控和炎症反应；明确防治的关键时间窗；从而揭示AMT对皮肤菌群结构和宿主细胞生物学功能的影响，阐明AMT对S.aureus抑制的作用机制。本研究将有助于理解皮肤共生菌对AD防治作用的功能，为开发新型皮肤共生菌防治AD提供新思路。

Human skin colonizes complex and diverse microbiota, and skin commensals microbiota participate in the formation of biological barriers and cellular metabolism, with immunomodulatory functions. At present, several coagulation-negative staphylococci (CoNS) strains that inhibit pathogen S. aureus in atopic dermatitis (AD) have been obtained through high-throughput screening. CoNS can prevent and treat AD, but the little is known about the specific mechanism. This project uses AD mouse model to co-culture CoNS-Staphylococcus aureus in vitro. RNA-seq, HPLC and Biolog are used to analyze how CoNS inhibits the growth of S. aureus through metabolic pathways and molecular mechanisms. The dermatitis wounds of AD mice are treated by autologous microbiota transplant (AMT). The structure and function of microbial flora are analyzed by metagenome and metatranscriptome, and the gene expression regulation of host cells and inflammatory response are detected. We should clarify the key time window of prevention and treatment, reveal the effect of AMT on the structure of skin flora and the biological function of host cells, and elucidate the mechanism of AMT on the inhibition of S. aureus. This study will help to understand the function of skin commensals microbiota in the prevention and treatment of AD, and provide new ideas for the development of new skin commensals microbiota to control AD.