油松LEAFY/NEEDLY靶基因筛选及其功能特异性研究

LEAFY是被子植物生殖发育调控网络中的核心调控因子，是整合内源与环境调控信号并向下传递引发生殖发育的节点。然而LEAFY同源基因在裸子植物生殖发育中的具体功能尚不十分清楚，特别是对其直接调控靶基因的筛选鉴定研究十分匮乏。在前期工作的基础上，本项目将以油松为材料，对其两类LEAFY同源基因（LEAFY ⁄ NEEDLY）的功能与作用机制进行解析：（1）筛选LEAFY同源基因的直接调控靶基因；（2）研究它们对油松BC类基因的调控及机制；（3）分析LEAFY与NEEDLY在油松生殖发育中的功能特异性。在本项目中，拟采用转基因表达谱分析、共表达网络分析、调控元件分析等新研究手段，旨在揭示以油松为代表的裸子植物LEAFY与NEEDLY在生殖发育调控中的具体功能，也为LEAFY同源基因在种子植物生殖进化中的地位与作用理论的发展提供新的数据支撑。

The LEAFY play a key role in the reproductive development regulatory networks in angiosperm plants, which integrate the reproductive development responses to the downward transmission of the endogenous and environmental signals. While the function of LEAFY homologous in the reproductive development of gymnosperms plants were still unclear, and few studies have identified the direct target genes of their. Based on our earlier investigations, we will investigate the function and mechanisms of the two LEAFY homologous, LEAFY and NEEDLY, in Pinus tabuliformis. First, we will identified the direct target genes of LEAFY homologous. Then we will explore the regulation mechanisms of the LEAFY to BC genes. Finally, we will further analyze the functional specificity of LEAFY and NEEDLY in the reproductive development of P. tabuliformis. In the course of the investigation, some new methods and techniques including gene expression profile analysis of transgenic cells, co-expression network analysis, regulatory element analysis will be used to determine the specific roles of LEAFY and NEEDLY in the reproductive development of gymnosperms plants, represented by P. tabuliformis. the results of this study will provide new insights into the roles and function of LEAFY homologous in the reproductive evolution of seed plants.