稻瘟病是水稻生产中最严重的真菌病害之一，对我国国民经济与粮食安全造成巨大威胁。抗病基因的发掘和利用是生产中防控稻瘟病最为经济有效和环保的手段。课题组前期研究克隆了水稻的广谱抗稻瘟病基因Piz-t，然而Piz-t介导的广谱抗病分子机制仍不明确。本研究前期工作以Piz-t为诱饵进行酵母双杂交筛选，发现Piz-t与一个新类型的转录因子PAT5相互作用。表达模式分析结果表明，PAT5在Piz-t介导的水稻与稻瘟菌非亲和反应中被显著诱导，而接菌结果显示pat5敲除突变体增强了对稻瘟菌的抗病性，表明PAT5在水稻抗稻瘟病PTI及Piz-t介导的ETI反应中均可能发挥重要作用。本研究拟进一步深入研究PAT5的时空表达模式及生化功能，分析其在水稻抗稻瘟病中的生物学功能，解析其与Piz-t的相互调控关系，从而揭示转录因子PAT5介导的抗稻瘟病分子机制。

Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most severe diseases in rice production which threatens China’s national economy and food security. The discovery and utilization of disease resistance genes is considered the most economical and practical approach to control rice blast.The broad-spectrum blast resistance gene Piz-t was cloned in the previous study,but the molecular mechanism of Piz-t-mediated broad-spectrum disease resistance remains unclear. In this study, Piz-t was used as bait for yeast two-hybrid screening.A novel transcription factor, PAT5, was identified as a Piz-t interaction protein. Expression pattern analysis showed that the expression of PAT5 was significantly induced in Piz-t-mediated resistance reactions. The pat5 knockout mutant showed enhanced resistance against M. oryzae, indicating that PAT5 may play a critical role in both PTI and Piz-t-mediated ETI in rice immunity. In this proposal, we will further analyze the temporal and spatial expression patterns as well as the biochemical functions of PAT5 to determine its role in rice blast resistance, along with its mutual regulation relationship with Piz-t. The accomplishment of this study may reveal the mechanism of Piz-t-mediated resistance and provide clues for effective control of rice blast in rice production.