经过长期研究，我们发展了蛋白质和多糖的绿色自组装路线。本项目旨在实现蛋白质-多糖复合体系的多功能化，以适应肿瘤诊断和治疗的要求。我们拟利用白蛋白-葡聚糖-叶酸共价复合物与抗肿瘤药物、Fe3O4纳米粒子、荧光探针自组装，高效制备具有磁和叶酸受体靶向的抗肿瘤复合纳米粒子。我们将通过细胞和动物实验调查复合体系在增加抗肿瘤能力和降低药物毒副作用方面的效果；我们还将通过荧光和磁共振扫描成像跟踪药物在荷瘤小鼠体内的分布情况以及肿瘤的诊断和治疗效果。

After long-term research, we developed a green strategy for self-assembly of protein and polysaccharide. For the purposes of tumor diagnosis and treatment, we are going to fabricate multifunctional protein-polysaccharide complex nanoparticles by self-assembly of BSA-dextran-folate conjugate, antitumor drug, Fe3O4 nanoparticles, and fluorescence probe. The antitumor activity and toxicity of the complex nanoparticles will be investigated by tumor cells and tumor-bearing mice. Magnetic resonance and fluorescence imaging of the nanoparticles will be investigated to evaluate the effects of tumor treatment and diagnosis.