DMD是一种进行性肌萎缩、腓肠肌假性肥大的X连锁隐性遗传病。患者12岁不能行走，20多岁死亡。目前认为基因治疗与干细胞治疗相联合是最佳治疗方法。我们前期的研究表明：移植治疗后肌肉中抗肌萎缩蛋白表达增多，mdx鼠的运动功能和肌肉病理改善、生命期限延长。但这些抗肌萎缩蛋白阳性肌细胞是供体来源的新生肌细胞还是回复肌纤维？如何对单个新生的神经肌肉接头进行鉴定？为了回答这些再生医学的基本问题，本研究拟（1）采用激光捕获显微切割技术，对脂肪干细胞移植的mdx鼠的同一肌肉切片，随机切下单个抗肌萎缩蛋白染色阳性的肌细胞、所支配的神经纤维和血管内皮细胞，再用单细胞PCR检测这些肌细胞、血管和神经是否含有供体的DNA成分，以确定是否来自供体干细胞的分化；（2）对这些鉴定后的单细胞再用RT－PCR法，分析其成肌、成血管和成神经因子的动态变化，从单细胞的角度探讨其再生的机制，这对于丰富再生医学理论有重要意义。

Duchenne muscular dystrophy (DMD) is the most common genetic muscle disease with characteristic of progressive degeneration and necrosis in skeletal muscle. Afflicted individuals often loss of the ability to walk independently by 12 years and die in their thirties,usually from respiratory and cardiac failure. Based on our previous research, we found that muscle regenerated with increased functional dystrophin, ameliorated motor function, improved pathology and extended life-span in mdx mice after stem cell transplantation. While, it is still not sure that whether those regenerated dystrophin positive myocyte were from donors. And how to identify the new regenerated neuromusclar junction? In order to solve those basic questions of regenerative medicine, in this study, we use Laser Capture Microdissection to separate the single cell of dystrophin positive myocyte ,as well as its connected vascular endothelial cell and innervated neuron. We will apply single cell PCR to sequencing their DNA compared with donor DNA. We will also use RT-PCR to detect their growth factors genes expression of those single cells, which will help us a better understand of those regenerative mechanism and also enrich regenerative medicine study.