炎性因子导致成骨相关miRNAs的异常表达，进而对下游成骨通路产生影响。在牙周炎和种植体周围炎的不同发展阶段,起调控作用的炎症相关miRNAs及其功能尚不明确。针对以上问题，构建狗的牙周炎和种植体周围炎实验动物模型，采用基因芯片和RT-PCR技术筛选不同炎症阶段差异表达的关键miRNAs。继而通过TNF-a和IL-1β刺激BMSCs模拟体外炎性微环境，验证炎症相关miRNAs的功能。并通过体外干预相关miRNAs的表达，检测在正常和炎症环境下BMSCs的成骨相关基因和炎症因子受体的表达情况。最后将含相关miRNA inhibitor的骨组织工程复合物运用于牙周炎和种植体周围炎的实验动物模型中，评估复合物能否有效促进牙槽骨炎症性骨缺损的修复。

Inflammatory factors can induce abnormal expression of miRNAs related to osteogenesis , and affect downstream osteogenesis pathways. In the different stages of development of periodontitis and peri-implantitis, inflammation associated miRNAs working as regulators and their functions are unclear. To solve above problems, we plan to construct the dog model of periodontitis and peri-implantitis and to screen key miRNAs differentially expressed during different stages of inflammation by using gene microarray and RT-PCR technique. Then to verify the expression levels of miRNAs associated with inflammation, we simulate inflammatory microenvironment in vitro through stimulating BMSCs by TNF-a and IL-1β. Also we will detect osteogenesis ability of BMSCs in both normal and inflammatory environment and the expression of inflammatory factor receptor through external intervention of related miRNAs expression. Finally we will construct bone tissue engineering compounds containing relevant miRNA inhibitor to be used in dog model of periodontitis and peri-implantitis to test whether the compounds can effectively control and treat the inflammatory alveolar bone defects.