麻醉中枢机制的研究是临床麻醉研究及用药的基础，隆朋是动物临床麻醉药物的组方基础，基于此，实验以大鼠为实验动物，隆朋设为对照、低剂量、中剂量和高剂量组，每组大鼠给药后设10min、30min、60min、90min、120min5个时间点，分别断头取大脑皮层、小脑、中脑、丘脑及海马等组织，利用RNAi技术对神经细胞内Ca2＋和cAMP信号转导系统中的关键调节蛋白CaMKⅡ、CaMKⅣ和PKC进行RNA沉默，分析CaMKⅡ-RNAi、CaMKⅣ-RNAi和PKC-RNAi与隆朋作用对p-CREB表达差异影响，在此基础上体外培养神经细胞，全面分析隆朋通过Ca2＋和cAMP途径对神经细胞核内CREB蛋白的磷酸化激活过程及细胞核内转录调控蛋白CREB对基因转录条控的机制。明确隆朋作用中枢神经系统后的麻醉作用机制，为今后指导临床更好应用药提供依据，也是今后开展相关药物研究的基础。

Anaesthesia center mechanism research is the clinical anesthesia and Medication foundation, xylazine is the animal basic clinical anesthesia combination junk. in view of this,In this study, wistar rats were selected as the experiment animals. And rats were divided randomly into four groups: the control group, the low dose group, the medium dose group and the high dose group. Animals in the experiment groups were treated with the same volume xylazine parenteral solution with different doses, such as low dose, the medium dose, or the high dose. Rats in the control group were administered with the same volume of physiological saline as the xylazine. The tissue samples of cerebral cortex, cerebellum, midbrain, thalamus and hippocampus in rats were individually collected at 10, 30, 60, 90min after the administration of xylazine. Effect of xylazine on differences expression of CREB mediated by intraneuronal Ca2+ and cAMP signal transduction in rat were investigated. And RNAi technique was used to silence key regulatory proteins CaMKII, CaMKIV and PKC of Ca2+ and cAMP signal transduction system of nerve cells. So that cellular level mechanism of CREB, the endonuclear transcriptional control protein on the gene transcription may be analyzed. Analyze the ctivate mechanism of xylazine on the phosphorylation of intraneuronal CREB mediated byCa2＋and cAMP and identify that which second messengers were selected after the anesthetics, which produced a marked effect on the endonuclear protein CREB, going into the nerve cells. So that it could accordance with it to utilize the anaresthetics, and it would be the base of medicine study in future.