课题以前期研制的山羊复合麻醉剂为基础，将复合制剂中隆朋、强痛宁、氟哌利多及复合制剂分别对山羊肌注给药，于注射后诱导期、麻醉中期、麻醉后期、苏醒初期、苏醒后期5个时间点分别取大脑皮层、小脑、中脑、丘脑及海马等脑区，研究单剂及复合制剂对山羊中枢信号转导系统、神经递质与突触体钙动力学、胞内c-fos和c-jun基因表达与神经细胞钙动力学的影响，阐明麻醉药物对其影响及这三大环节之间的相互关系，明确山羊复合麻醉剂组方中每一种药物的中枢作用机制、作用部位，分析单剂之间是否具有叠加区域、盲区补充覆盖及作用机制的协同性。找出对组方改进的依据，同时也为下一步其他反刍动物复合麻醉的研究奠定基础。

Based on the combined anaesthetic for goat developed by our laboratory，the combined anaesthetic and its components xylazine, qiangtongning, droperidol were injected into muscle of goat respectively, then the cerebral cortex, cerebellum, deutocerebrum, cerebral ganglion and cornu ammonis were collected during cornu ammonis, intermediate stage, later period of anesthesia, and initial stage of palinesthesia, later stage of palinesthesia. The effects of each components and combined anaesthetic on center signal transduction system, neurotransmitter and synaptosome calcium dynamics, and expression of c-fos and c-jun of intra-cellular, dynamics of calcium for nerve cell were studied. To interpret the relationship among those three component elements and effect of junk on above system. Then identify the effects mechanisms, site of action of every components of combined anaesthetic for goat. To analyze if there existed superimposing area, supplementary overlap for dead zone, cooperativeness of action mechanism among every components. Eventually find the evidence to modified combination of anaesthetic, and establish foundation for research of other combined anaesthetic of ruminant.