束缚应激致大鼠杏仁核及心肌细胞凋亡的分子机制研究

现行法律规定，严禁以持续捆绑或限制体位等虐待方法对嫌疑人逼取口供。在法医检案中经常遇到受害人在遭受持续捆绑或限制体位等束缚情形下，生命重要器官损伤或死亡的案件。由于单纯此类损伤本身不足以导致死亡，甚至没有明显肉眼可见的外伤，使死因难以确定。因此揭示此类损伤或死亡的病理分子机制是法医病理学亟待解决的前沿性科学技术问题。我们前期研究发现及相关文献报道，束缚应激作为应激原可引起机体强烈的应激反应，我们还观察到大鼠海马、肝、肾、肺等多个组织器官细胞发生凋亡。目前，应激所致的中枢神经系统及心脏损害和功能障碍的司法鉴定案件逐年增多，其损害机制是否与应激引起神经元和心肌细胞凋亡有关尚不清楚。本项目拟对应激引起杏仁核神经元和心肌细胞凋亡的分子机制进行系列研究，对应激诱导凋亡的分子病理学变化进行探讨，为束缚应激参与的机体损害案件的鉴定提供依据，为有效执行《刑事诉讼法》关于严禁刑讯逼供的规定提供科学技术支持。

In the current legal rule, it is strictly prohibited to sustained bind or limit position abuse methods to extract confessions. During the forensic practice, it is often occurred that the important vital organs of the victim injury or death under the sustained binding or limit position environments. Because pure injury alone is not sufficient to cause death, even there is no obvious visible injuries, it is difficult to determine the cause of death. Therefore, it is a frontier scientific and technological problem to reveal the exact pathological and molecular mechanism of injury and death in forensic pathology science. Our previous study and the relevant leteratures found that as a stressor, restraint stress can induce stress response. Moreover, we also found that restraint stress could cause the apoptosis of some tissues and organs, such as hippocampus, liver, kidney and lung et al. At present, the judicial identification cases of the central nervous system and heart damage and dysfunction caused by stress is increasing year by year. It is still unknown that the relationship between the damage and the amygdala and myocardial cell apoptosis caused by stress response. The present project intends to carry out series of studies on the specific mechanism of amygdala and myocardial cell apoptosis induced by stress and explore the molecular pathology changes of apoptosis induced by stress. Furthermore, our study will provide the scientific basis for identification of damage cases involved in stress and the necessary technical supports for effective executing the “Criminal Procedure Law” on the prohibition of torture to extract confessions.