轮奸案混合DNA分析关键技术基础研究

对轮奸案混合DNA检材如何准确分型？以进行有效正确的个体识别，是国际上未解的法医学重大技术难题。.本研究选择轮奸案中最常见的两名嫌疑人和受害人构成的三人混合DNA样本为研究对象，构建轮奸案混合DNA实验模型；通过最大等位基因数估计和最大似然率估计两种方法对来源个体数进行推断；采用多元回归分析、限制性表型组合排列分析、等位基因矢量分析的统计方法对混合DNA分型的量化信息进行评估分析，以期将两名嫌疑人DNA分型从轮奸案混合DNA分型中分离，与受害人DNA分型比对后确定嫌疑人各自的DNA分型，并对此时分离出的DNA证据强度进行概率计算，确定该分析结果的把握度；通过对实际案例的检验，将分析结果与实验模型进行比较分析，观察和统计轮奸案混合DNA分析过程中出现的主观性和偏差，确定所建立分析模型的适用范围，为轮奸案侦破提供技术支持。

How to accurately points genotype of gang rape caese DNA samples? To identify the right individual effectively is the international unanswered forensic key technical problems..This study chooses three person mixed DNA that have two suspects and one victim as the object of research, constructing experimental model of mixed DNA from gang rape case. Using two methods of the maximum allele count and maximum likelihood estimator estimates the number of contributors. Selecting and combining three statistical methods of multivariate regressions analysis, restricted permutations of genotype combination and allelic vector analysis to evaluate quantitative information of mixture DNA profiles, hope to separate two suspects' DNA profiles from this mixtue. After compare to victim DNA profile, we can confirm these suspects' DNA profiles, then calculate the strength of DNA evidence for confidence of the analysis results. Through application verifications of actual gang rape cases, the results are analyzed comparatively with results of experimental model. Subjectivity and bias of this constructed analysis model in this research during analysis procedure should be observed and analyzed to ensure the scope of application, so as to provide technical support for gang rape cases.