本项目采用超临界CO2-薄膜蒸发法制备具有不同微观结构的生物靶向的micro-RNA荧光脂质体。采用物理化学的研究方法、热力学的研究手段、计算机模拟和现代测试技术，探讨这些荧光脂质体可控自组装过程及其调控规律；研究不同结构不同功能荧光micro-RNA脂质体的制备、结构、宏观性质和靶向功能。以乳腺癌的肿瘤细胞和正常细胞为研究对象，荧光脂质体将micro-RNA运送到治疗干预靶点，通过micro-RNA脂质体与目标膜发生的跨膜、刺激响应开关控制释放micro-RNA等过程，研究micro-RNA荧光脂质体与活体细胞之间相互作用、宏观性质与微观结构和功能之间的关系，揭示micro-RMA脂质体的微观结构对其靶向性和生物利用度的影响及其规律。该研究的结果将为肿瘤治疗提供重要信息，为恶性肿瘤的早期诊断提供新的靶标。

A series of fluorescent micro-RMA liposomes with biological target will be prepared by combination method of supercritical CO2 and thin film evaporation. Using physical chemistry method, computer simulation and the modern detection technology, controllable self-assembly process of fluorescent liposomes will be studied. The preparation, structure and macroscopic properties and target function of micro-RNA liposomes will be revealed. The process of trans-membrane, fusion, and stimulus response switch control release of the micro-RNA will be probed in the biological system of breast tumor cells and normal cells. The relationship between the microstructure, properties and function will be explored. The interaction between the micro-RMA liposomes and biological molecules will be revealed. The interaction between the micro-RNA liposomes and living cells, microstructure, and the relationship between the macroscopic properties and microscopic structure and function will be discussed. The targeting and tumor suppressors influence on the microstructure will be uncovered. The research results will provide important information for tumor treatment, new targets for the early diagnosis of malignant tumor.