集诊断、靶向治疗一体的多功能载药体系的制备、性质、功能及其相互作用热力学

荧光示踪纳米材料在生物医学领域中具有广阔应用前景，受到广泛关注。本课题采用超临界CO2方法制备一种新颖的具有可逆荧光特性的纳米PDA-脂质体。采用热力学的研究思路和方法获得这些新型复杂体系中宏观热力学参数及其变化;使用现代仪器和测试手段研究它们的形貌、性质与功能;利用计算机模拟和现代测试技术获得微观结构及其变化规律。采用物理化学和生物结合的研究的方法，从分子层次探讨PDA-脂质体在生物体系中的作用和功能，考察这些相互作用可能导致的生物功能和细胞毒性。研究结果将揭示PDA-脂质体的制备、性质、微观结构、功能之间的关联，以及PDA-脂质体与生物体系的相互作用的宏观性质与微观结构和功能之间的关系，为荧光示踪脂质体在生物体系中的应用提供理论依据。

Fluorescent tracer nano-materials have broad application prospects in the biomedical field, attracted widespread attention. A novel nano-PDA-liposome with reversible fluorescent characteristics will be synthesized by using supercritical CO2 method in this project. For this complex system, the macroscopic thermodynamic parameters and their changes will be investigated by using thermodynamics methods. The morphology, property and function will be obtained by modern instrumentation and testing techniques. The microstructure and its variation will be obtained by computer simulation and modern testing techniques. The role and functions of the PDA-liposomes in biological systems will be explored at the molecular level, and these interactions may lead to biological function and cytotoxicity will be also studied. The findings will reveal the PDA-liposomes, the association between the nature of micro-structure, function, and PDA-liposomes and the interaction of biological systems the relationship between macroscopic properties and microscopic structure and function for the fluorescence tracer liposomes in the biological system to provide a theoretical basis.