云南鱼类单殖吸虫的种类组成、宿主特异性及其系统发育研究

宿主特异性及协同进化使单殖吸虫成为研究寄生现象起源和发展、寄生虫与宿主协同进化理想的实验范例。单殖吸虫分子系统发育研究为揭示和重建脊椎动物宿主的系统起源进化提供了新颖的视角。云南具有丰富的鱼类及单殖吸虫多样性，地方性分布的鱼类、土著鱼类单殖吸虫对于揭示寄生虫和宿主的协同进化具有有别于我国其他区系的独特性。本项目调查研究云南鱼类寄生单殖吸虫本底资源及分布情况，明确云南鱼类单殖吸虫的种类组成、宿主特异性及其区系特征；基于rDNA（ITS1或28S rDNA）序列重建系统发育，揭示鱼类-单殖吸虫协同进化关系。该研究虽属于基础理论研究范畴，但研究成果将对吸虫志做出重要补充，充分体现云南地理区位所致的物种多样性优势，为单殖吸虫系统发生呈现东南亚（中国）的进化世系证据，为进一步揭示寄生虫与宿主之间的协同进化提供新的、必不可少的研究例证，具有重要学术意义。

In view of the host specificity and coevolution between Monogenea-fish associations, Monogenea present strikingly special relationship and idealized experimental paradigm in the field of origin and development of parasitism. The molecular phylogeny of Monogenea leads to new insights on the origins of their early vertebrate hosts while simultaneously phylogenetic relationships of hosts were reconstructed and inferred from the phylogeny of their monogenic flatworms based on genetic molecular data. The biodiversity of class Monogenea are widely diverse with their host fishes of Yunnan Province,where has repeatedly been ranked high in terms of species diversity, such as endemicity and indigene species fishes. Endemically distributed species can better represent the characteristics of fauna and inital radiation and coevolution with Monogenea-fish associations. The present application focus on monogenic flatworms that parasitic in fishes of Yunnan Province. New species and composition will be discribed by numerical taxonomy methods according to morphologic characters. The level of host-parasite specificy and characteristics of fauna will be ascertained.Phylogenies of host and parasite between genera will be indicated from genetic molecular data of 18S rRNA ITS1 gene sequences to examine host-parasite coevolution. The basal research application will enrich the fauna sinica platyhelminthes, and adequately reveal the unique biodiversity and superiority of geographical district of Yunnan province.The research will provide a potent exemplification of southeastern Asian to primarily demonstrate the mechanism of historical biogeography and coevolution of Monogenea-fish associations. The data of molecular phylogeny will distinctively infer the long-standing coevolution relationship between fish and monogenic parasite.