基于线粒体基因组云南-东南亚跨境河流鲤科鱼类单殖吸虫亲缘地理及其与宿主共系统发育研究

单殖吸虫宿主特异性显著，与宿主协同进化关系尤为密切，其适应辐射和进化受到宿主进化和分布的影响。云南鱼类单殖吸虫多样性丰富，地方性分布和土著鱼类的单殖吸虫对揭示协同进化具有有别于其他区系的独特性。本项目拟以云南--东南亚跨境河流为研究的特定地理区域，土著鲤科、鲤科和鲤形目三个不同尺度鱼类类群及其寄生单殖吸虫代表物种为特定分类单元，接续开展鲤科鱼类单殖吸虫分类、亲缘地理及共系统发育研究。综合形态学特征和线粒体基因组特征，研究单殖吸虫的区系、宿主特异性、鉴定性状的种间变异和种内变异，分析相似性状的特征演变及共系统发育关系，解析物种丰富度、宿主特异性与宿主亲缘地理学的内在联系，为揭示单殖吸虫及鲤科宿主的物种演化、多样性及地理分布格局的形成提供东南亚的新的数据和研究范例，也为考证鲤科鱼类宿主的分类体系及起源进化提供独特证据。

Class Monogenea exhibiting a high degree of host-specificity, is expected to be intimately associated with the co-evolution of their host. The adaptive radiation and evolution of monogeneans is shaped by the evolutionary history and distribution of such vertebrate hosts. The biodiversity of Monogenea are widely diverse with their host fishes of Yunnan Province. The relationship between monogeneans and their endemicity species fishes or distributed species fishes can be better for revealing the specificity characteristics of co-evolution within parasite-host association than other fauna. The present application focus on the taxonomy, phylogeography and co-phylogenetic relationship of specially designated geographical research areas and specific taxonomic category of Monogenea of Cyprinidae fishes complementally. Monogenea and their host fishes including endemicity species, Cyprinidae and Cypriniformes in Yunnan-Southeast Asia transboundary rivers are taken as three different scale groups of research subjects. Evolution of similar characteristics and co-phylogenetic relationship are inferred from the fauna, host-specificity, intraspecific and interspecific variations of identify traits of monogeneans through a combined morphological features and basic characteristics of mitochondrial genome sequences of parasites and their host fishes. The correlation biological mechanism among species richness, host-specificity and phylogeography will be indicated from the topological congruence of Monogenea-Cyprinidae association. The expected results of this application will provide a potent experimental paradigm and the unique evidence in the field of Southeast Asian, and primarily demonstrate the biological mechanism of species evolvement, biodiversity and historical biogeography of Monogenea-Cyprinidae association. Furthermore, it is of great scientific significance to supply the novel and specific evidences for the taxonomy and original evolution of Cyprinidae fish hosts of Southeast Asian.