水稻种子休眠性是关系到稻米品质和稻种质量的重要农艺性状，是受主基因+多基因控制的数量性状，且受环境条件的影响，迄今对调控该性状的分子机制尚不清楚。在前期研究中，已构建了以南粳35为受体、N22为供体的，携带qSdn-1的近等基因系，利用近等基因系构建的次级分离群体，对qSdn-1进行了精细定位，将其限制在29.8kb范围内，含3个完整的ORF。本研究拟在此基础上，结合生物信息学的方法，分离克隆qSdn-1基因。对此区间进行测序，通过序列分析、同源性比较、表达谱分析、酵母双杂以及转基因研究进行功能验证，结合前人已克隆的水稻种子休眠性相关基因（（包括OsDOG1、OsSdr4、OsVP1等）），利用近等基因系、转基因材料及突变体材料，分析qSdn-1与其他控制休眠性基因间的关系，阐明其在水稻种子休眠和萌发控制途径中的作用机制，同时获得紧密连锁或共分离分子标记为水稻适宜休眠性的分子育种提供基础。

Seed dormancy in rice, an important agronomic trait related to rice quality and seed quality, is a quantitative trait controlled by the major gene and multiple genes, and is affected by environmental conditions. To date, the molecular mechanism regulating this trait is not yet understood. In the preliminary study, the applicant had already constructed near-isogenic line carrying the major seed dormancy QTL qSdn-1 (Nanjing 35 as receptor and N22 as donor). Then a secondary segregation population built by near-isogenic line is utilized for high-resolution mapping and the qSdn-1 was localized to a 29.8 kb region containing three intact open reading fragments.In this study, based on the method of bio-informatics and previous research, qSdn-1 gene will be isolated and proceed for function verification by sequencing, blasting, expression file and yeast two-hybrid assay, and transgene for overexpression and RNAi of candidate genes. The relationship between qSdn-1 and known seed dormancy genes such as OsDOG1、OsSdr4、OsVP1,and traits related to physiological processes of seed dormancy and germination will be analyzed using near-isogenic lines, transgenic plants and mutuants. These results will help us to understand the molecular mechanism of qSdn-1 in rice seed dormancy and preharvest sprouting resistance, as well as the metabolic network controlled by qSdn-1, and to promote the breeding program of PHS resistance by molecular assitant seletion in rice.