梯棱羊肚菌是近年来规模化栽培的珍稀食用菌，但其产量不稳定。菌核是其子实体发育的重要阶段，产菌核能力和数量是栽培中衡量菌种质量的重要标准。项目前期研究发现活性氧（ROS）调控其菌核形成，高ROS区细胞老化，低ROS区形成菌核，两区域细胞中相关基因表达水平不同，表明不同氧化压力下细胞具不同的分化命运，但相关作用机制资料匮乏。本项目拟以响应ROS诱导信号转导途径中的转录因子MSN2为切入点，通过磷酸化水平、亚细胞定位检测，揭示MSN2感受ROS诱导信号转导与其修饰水平和定位间的关系；ChIP-Seq检测其靶基因，结合MSN2过表达和干扰菌株表型观察、转录组分析靶基因表达水平等，揭示MSN2在细胞中响应不同ROS强度诱导信号转导的分子机制，从而阐明其调控菌核发育的功能。本研究为深入阐明梯棱羊肚菌菌核发育机制提供基础，更为解决人工栽培中相关科学问题提供理论依据，预期研究具有重要的科学意义和实践价值。

Morchella importuna are prized edible mushrooms and the outdoor cultivation of morels has succeeded in recent years, but the production is unstable. Sclerotium is an important phase in Morel’s life cycle and the amount of sclerotium is the most important quality standard of the spawn. Our previous studies showed that sclerotium formation regulated by reactive oxygen species (ROS), cells in the high ROS stress area gradually aged, and cells in the low ROS stress area formed sclerotia. Moreover, ROS related gene’s expression level in two areas were different, showed that cells in different ROS stress with different of morphogenesis, and the ROS play an important role in regulation of sclerotia formation, but its mechanism is not clear. In this project, we focused on MSN2, which is a transcription factor in the ROS induced signal transduction pathway. Applied with phosphorylation level and subcellular localization detection, to discover the mechanism of the MSN2 activated by ROS signal. ChIP-Seq performed to detection of MSN2’s target gene, combined with MSN2 overexpression and interference strain phenotype observation, transcriptome analysis of target gene expression level, revealed the molecular mechanism of MSN2 in response to the signal transduction induced by different ROS intensity, so as to clarify its functional mechanism of regulating sclerotia development. This study provides a basis for further elucidation of the sclerotia development mechanism of Morchella importuna and a theoretical foundation for solving relevant scientific problems in artificial cultivation. It is expected that the study will have important scientific significance and practical value.