Mating-Type Genes Play an Important Role in Fruiting Body Development in Morchella sextelata.
Mating-Type Genes Play an Important Role in Fruiting Body Development in Morchella sextelata.
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交配型基因在羊肚菌子实体发育中发挥重要作用
DOI：
10.3390/jof8060564
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发表时间：
2022-05-25
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
真正的羊肚菌（羊肚菌属）是一种可食用的蘑菇，由于其丰富的营养和独特的外观，在世界范围内具有重要的商业价值。近年来，室外种植在中国已经实现并大规模扩展。然而，人们对羊肚菌子实体发育的机制了解甚少。本研究主要研究了交配型基因在子实体发育中的作用。不同交配型菌株培养的子实体在外观上没有差异，但MAT1-1菌株的子实体的子囊和子囊孢子有轻微畸形。结合体型基因及其靶基因的转录水平揭示了子囊菌真菌的调控机制是保守的。RNA干扰对mat1-2-1的沉默证实了mat1-2-1在无性繁殖阶段对其靶基因的直接调控作用。当与MAT1-1或MAT1-2单交配型菌株的菌种培养时，在菌丝和分生孢子样品中只检测到一个对应的交配型基因，而在原基、菌毛和茎柱样品中均检测到MAT1-1 -1和MAT1-2 -1。了解交配型基因在六分木犀子实体发育中的作用，有助于了解六分木犀的生命周期，为人工栽培提供依据。
True morels (Morchella spp.) are edible mushrooms that are commercially important worldwide due to their rich nutrition and unique appearance. In recent years, outdoor cultivation has been achieved and expanded on a large scale in China. However, the mechanisms of fruiting body development in morels are poorly understood. In this study, the role of mating-type genes in fruiting body development was researched. Fruiting bodies cultivated with different mating-type strains showed no difference in appearance, but the ascus and ascospores were slightly malformed in fruiting bodies obtained from the MAT1-1 strains. The transcript levels of mating-type genes and their target genes revealed that the regulatory mechanisms were conserved in ascomycetes fungi. The silencing of mat1-2-1 by RNA interference verified the direct regulatory effect of mat1-2-1 on its target genes at the asexual stage. When cultivated with the spawn of single mating-type strains of MAT1-1 or MAT1-2, only one corresponding mating-type gene was detected in the mycelial and conidial samples, but both mat1-1-1 and mat1-2-1 were detected in the samples of primordium, pileus, and stipe. An understanding of the mating-type genes’ role in fruiting body development in M. sextelata may help to understand the life cycle and facilitate artificial cultivation.
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影响因子：
3.3
作者：
Hanson SJ;Wolfe KH
通讯作者：
Wolfe KH
DOI：
10.1016/j.fgb.2012.05.004
发表时间：
2012-07
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
Wang Z;Kin K;López-Giráldez F;Johannesson H;Townsend JP
通讯作者：
Townsend JP
羊肚菌产量与土壤微生物多样性和均匀度相关
影响因子：
3.7
作者：
Tan H;Liu T;Yu Y;Tang J;Jiang L;Martin FM;Peng W
通讯作者：
Peng W
影响因子：
6.4
作者：
Rodenburg SYA;Terhem RB;Veloso J;Stassen JHM;van Kan JAL
通讯作者：
van Kan JAL
影响因子：
16.8
作者：
Murat, Claude;Payen, Thibaut;Martin, Francis M.
通讯作者：
Martin, Francis M.