基于光谱解调技术的荧光微球编解码方法研究

近年来，液相生物芯片技术在基因测序、蛋白分析、医疗诊断等领域显示了巨大的应用前景和研究价值。液相生物芯片技术的核心是功能性荧光编码微球，因此，高质量荧光编码微球的制备及解调具有重要的研究意义。目前，液相生物芯片的检测主要基于流式细胞技术。检测时要求同种编码微球具有均一的亮度，对荧光物质的光谱重叠区域用经验补偿法进行调节，影响了检测的准确度，也增加了微球制备的难度。.在该项目中，我们首先制备出高亮度荧光编码微球并收集荧光信号，使用一种基于光谱解调技术的荧光微球编解码方法对微球荧光信号进行函数叠加运算，由最小二乘法求得光谱中各函数基的系数；用“系数归一化”方法最终实现对微球的解码。同时由此提出波形相关的解调方法，利用波形码的相关性运算完成对微球的编解码。这两种解调方法在荧光微球编解码中可能有较好的应用前景。

In recent years, liquid bio-chip technology in gene sequencing, protein analysis, medical diagnostics and other areas shows great prospect and research value. The core of liquid bio-chip technology is the functional fluorescent coded microspheres, therefore, the preparation and demodulation of high-quality fluorescent coded microspheres have important significance..At present, the detection of liquid bio-chip is mainly based on flow cytometry.The same kind of coded microspheres are required to be a uniform luminance when detecting,and the empirical compensation algorithm is used to deal with the spectral overlap of different fluorescent dyes, which cause high constraints on the accuracy of detection and increase the difficulty of preparation..In this project, we prepare high-brightness fluorescent microspheres and collect the fluorescence, use a decoding method based on the technology of spectrum demodulation to finish signal superposition.The coefficient of each function basis is obtained through the least-square method. The decoding of microspheres is achieved by the normalization of coefficient. Meanwhile, the Waveform Correlation demodulation is proposed. These two kinds of demodulation method to fluorescent microsphere are expected to have good application prospects.