糖尿病勃起功能障碍（DMED）是勃起功能障碍常见类型之一，阴茎血管内皮损伤是DMED发生的始动环节和病理生理基础。其中，CaSR/Gq--PLC--PKC通路的过度激活是糖尿病血管内皮损伤的关键因素。前期研究证实活血通络方剂“活血通络起痿汤”治疗DMED疗效优于传统补肾壮阳方剂，具有改善血管内皮功能的作用。“水蛭-蜈蚣”是临床上治疗ED经典的活血通络药对，也是“活血通络起痿汤”的核心药对。因此，探索“水蛭-蜈蚣”是否通过调控上述通路改善DMED血管内皮功能从而发挥与全方相似的作用是研究的关键问题。本研究以CaSR/Gq--PLC--PKC通路为切入点，构建DMED大鼠模型，用“水蛭-蜈蚣”药对进行干预，观察大鼠勃起功能、血管内皮形态学与损伤标志物、CaSR/Gq--PLC--PKC通路蛋白、mRNA的表达情况，探索该药对治疗DMED可能的分子机制，为活血通络法治疗DMED提供科学依据。

Diabetes mellitus erectile dysfunction(DMED) is a common complication of ED, penile vascular endothelial injury is initiating links and pathophysiological basis of DMED. Excessive activation of CaSR/Gq--PLC--PKC pathway is a key factor in diabetic vascular disease. Early studies of "Huoxue Tongluo Qiwei soup" to treatment DMED achieved a significant effect which is better than “nourishing kidney and tonic yang medcine”, which has the effect of improving endothelial function of blood vessels. "Leech-centipede" is the key medicines for promoting blood circulation and removing blood stasis, which are also core herbs in "Huoxue Tongluo Qiwei soup". Therefore, to explore whether it by regulating the above pathway to improve endothelial function and then play a similar role with the whole party is a key problem to be solved. With this study, we focus on CaSR/Gq--PLC--PKC pathway, induce DMED rat models, then measured the rats erectile function, vascular endothelial morphology adn injury markers, the expression level of protein and mRNA in CaSR/Gq--PLC--PKC pathway to explore a possible molecular mechanism for the herbs treatment and provide a scientific basis for application of DMED with promoting blood circulation and activating collaterals.