Zn-nHA/PLGA/FBHA复合材料通过调控Th17/Treg细胞平衡影响种植体周围炎骨微环境促进骨缺损修复的研究

种植体周围炎骨微环境具有炎性状态迁延、成骨破骨功能失衡的特点，对骨缺损修复进程产生不良影响。Th17/Treg细胞平衡是种植体周围炎骨微环境的重要调节因素，而研究发现锌离子在调控Th17/Treg细胞平衡中发挥重要作用。针对“如何调控种植体周围炎骨微环境并促进骨缺损修复”这一关键科学问题，本项目以调控Th17/Treg细胞平衡为新型骨替代材料研发的切入点，结合申请人前期聚焦的离子植入在调控骨微环境中的作用，首先评估锌离子通过影响Th17/Treg细胞平衡调控骨微环境的作用及其机制。进而合成锌掺杂纳米羟基磷灰石（Zn-nHA），通过静电纺丝及冰冻切片技术制备Zn-nHA/PLGA混纺短纤维，涂覆在申请人研发的具有血管化成骨效能的氟化生物骨源羟基磷灰石(FBHA)支架，构建Zn-nHA/PLGA/FBHA复合材料，以调控种植体周围炎骨微环境促进骨缺损修复，为种植体周围炎骨缺损的治疗提供新思路。

The bone micro-environment with peri-implantitis is characterized by sustaining chronic inflammation and unbalanced osteogenesis/osteoclastic activity, which severely impaired the bone regeneration process. The balance of Th17 cells and Treg cells played vital roles in the micro-environment modulation of peri-implantitis. Zinc ion have been found to induce T cells differentiate into Treg cell and inhibit the differentiation of Th17 cell, thus mediating the balance of Th17/Treg cell. Aiming at addressing the scientific problem “how to improve bone regeneration by regulating the bone micro-environment of peri-implantitis”, this project raises a novel research strategy of bone substitute material which emphasizes the modulatory properties of Th17/Treg cell balance. Combing our previous researches which focused on the osteo-immunomodulation of implanted metal ion, we will primarily evaluate the modulatory effect and underlying mechanism of gradient concentration zinc on Th17/Treg cell balance and its influence on bone micro-environment. Then, we will construct a sustained release zinc system (Zn-nHA/PLGA/FBHA) based on nHA and FBHA by electrospinning and cryo-sectioning technique, and it was expected that the new material will have regulatory function on bone micro-environment modulation and promote vascularized bone regeneration for the treatment of peri-implantitis.