骨软骨损伤目前临床手段疗效有限，原因之一就是骨软骨复合组织结构复杂，软骨与软骨下骨之间的连接结构再生困难。我们前期自主研发的胶原基高强度力学材料能有效支持关节软骨再生，同时也成功搭建了基于连续液面提取技术（CLIP）的3D打印平台。因此，我们提出在系统解析骨软骨复合组织3D结构和细胞外基质成分（ECM）的基础上，研究开发智能响应生物材料和运用CLIP 3D生物打印技术进行骨软骨复合组织的精准再生研究，包括：1）运用同步辐射等技术解析骨软骨复合组织ECM成分，建立其三维移行结构和ECM成分图谱；2）基于ECM成分解析进行智能响应生物材料的研发；3）基于三维移行结构和CLIP 3D生物打印技术一体化构建骨软骨复合组织再生支架；4）体内评估骨软骨复合组织支架对于骨软骨缺损修复的效应。本研究发现将系统揭示骨软骨复合组织连接处的结构与ECM成分，并在技术上提供骨软骨复合组织精准再生的新材料和新手段。

Clinical treatment of osteochondral injury is still unsatisfactory. One of the reasons is that the structure of osteochondral tissues is complicated, and the transitional structure between cartilage and subchondral bone is difficult to regenerate. In our group, we have developed collagen-based biomaterials with high strength can effectively support articular cartilage regeneration, and also successfully built up a 3D bioprinting platform based on continuous liquid interface production (CLIP). Therefore, we propose to do the following study based on our previous work: 1) To analyze the ECM components of osteochondral tissues with high-throughput proteomics and other methods and establish its precise three-dimensional structure and ECM component mapping; 2) Based on ECM component analysis, to develop intelligent response biomaterials with collagen-based materials and silk; 3) Based on three-dimensional structure, to construct multi-layered osteochondral scaffold with CLIP 3D bio-printing technology; 4) To evaluate the effect of printed scaffold on osteochondral defect repair. After this study, we will systematically find out the structure and ECM components of the osteochondral tissue, and provide new biomaterials and new approach for the accurate regeneration of osteochondral defect.