在大规模核与辐射突发事故的应急医疗救助中，对伤员进行快速准确的全身生物剂量评估和重要组织脏器损伤程度评估是正确后续处理的关键环节。传统的生物剂量测定法存在耗时、费力及非均一性等问题；因此，有必要探索更灵敏、可靠、高通量的便携生物剂量估算和组织辐射损伤评估平台。本项目拟利用代谢组学、脂质组学等多组学技术，在全身照射（TBI）和局部照射（PBI）两种模式下，明确血浆、尿液、和重要组织中代谢分小子或脂质分子对电离辐射的剂量-响应和时间-响应规律，并结合生物信息方法，筛选出电离辐射敏感组合标志物；用生物统计学方法进行数据挖掘与整合，搭建基于高通量、便携液相悬浮芯片技术的辐射损伤生物剂量评估体系。本项目将为建立快速、灵敏、便携的辐射生物剂量检测平台和机体重要组织辐射损伤评估系统提供实验依据和理论指导。

After a large-scale nuclear and radiation accidents, rapid and reliable radiation dose estimation of possible exposed individuals is one of key links to emergency medical assistance. Moreover, a rapid, sensitive and accurate assay for assessing the severity of the critical organ systems. Conventional biodosimetry methods are very labor-intensive, time-consuming and heterogeneous. Therefore, it is very important to explore the more sensitive, accurate and throughput methods of biodosimetry and assessment of tissue injury. In this project, metabolomics and lipidomics are used to analyze the rules of dose-response and time-response of metabolome and lipids in plasma (or urine) and in critical tissues to select radiosensitive molecular markers in total-body irradiation(TBI) and partial-body irradiation (PBI) exposures. On that basis, a bioinformatics method for multiple data analysis is exploited to further refine and forecast the panel of radiosensitive markers, and then a chip system based on liquid suspension array is built and validated. The implementation of the project will provide experimental evidence and theoretical guidance for the development of rapid, sensitive and accurate radiation biodosimetry and critical organ systems.