神经突触膜蛋白Neurexin/Neuroligin之间的相互作用调控神经突触的形成、成熟及可塑性，从而影响神经元之间准确的信号传递。我们初步发现，生物力与糖基化修饰可以动态调控 Neurexin/Neuroligin之间相互作用的动力学参数，影响神经突触的生物学功能；但是，其力学-化学耦合调控的分子机制仍不清楚。本项目拟整合生物力学、分子生物学、神经生物学、结构生物学和计算生物学等多种研究手段，在单分子水平深入研究Neurexin/Neuroligin之间的动态相互作用，揭示生物力耦合糖基化修饰通过膜蛋白构象或空间取向变化动态调控相互作用特性，为膜蛋白调控神经突触功能提供了一种全新的分子机制。在此基础上，提出力学-化学耦合动态调控膜蛋白相互作用及其生物学功能的内在规律，为神经、免疫及肿瘤等生物学科提供了一种崭新的研究视角。

The Neurexin/Neuroligin interaction regulates the formation, maturation and plasticity of neural synapses, thereby affecting accurate signaling transmission between neurons. We preliminarily found that mechanical force and glycosylation modification could dynamically regulate the kinetic parameters of the Neurexin/Neuroligin interaction, thus affecting the synaptic function. However, the molecular mechanism of its mechanical-chemical coupling regulation is still unclear. This project intends in-depth study on the dynamic Neurexin/Neuroligin binding kinetics through integrating multi-discipline research methods, such as biomechanics, molecular biology, neurobiology, structural biology and computational biology, revealing a novel molecular mechanism that the coupling between mechanical force and glycosylation modification dynamically regulates Neurexin/Neuroligin binding kinetics and their function through conformation and orientation changes. On this basis, the intrinsic law of mechanical-chemical coupling regulation on transmembrane protein interaction and its biological functions might also provide a novel research perspective for neurobiology, immunology and tumor biology.