组蛋白甲基化、乙酰化等修饰水平是细胞表观遗传活跃程度的一个重要指标，在基因表达和功能发挥中起重要作用。雌激素受体被认为在骨质疏松中起着关键作用，雌激素替代疗法被广泛用于骨质疏松病人的治疗。最新研究发现，多个组蛋白修饰酶通过激活雌激素受体发挥功能，但表观遗传因子在骨质疏松发生、发展中的作用尚不清楚。本研究：①拟采用基因表达谱分析技术（cDNA Microarray）、染色体免疫共沉淀、免疫沉淀（chip-seq）、基因沉默（siRNA）和超表达等技术，研究组蛋白去甲基化酶JMJD2蛋白家族在骨形成中作用和补肾中药的关系;②借助骨质疏松动物模型和病人临床样本，研究组蛋白去甲基化酶JMJD2、组蛋白甲基化与骨质疏松之间的关系，以及组蛋白去甲基化酶JMJD2在补肾法治疗骨质疏松中的作用。探明组蛋白甲基化修饰酶在骨形成中的分子作用机理，以及与骨质疏松的关联性，为中药治疗骨质疏松提供新思路和理论依据。

Histone methylation,acetylation etc modified levels are key indicators of of cellular epigenetic activity，and play an important role in the gene expression and function.also,estrogen receptors(ERs) are believed to be vital in osteoporosis, Therefore,estrogen replacement therapy is widely used for the treatment of osteoporosis patients.Furthermore,Recent studies found that multiple histone -modifying enzymes function through activing the estrogen receptor,but epigenetic factors in the role of incidence and development of osteoporosis is unclear. So, In present research,①we employ cDNA Microarray、Chromatin immunoprecipitation、chip-seq、siRNA and gene overexpression technologies as investigative tools,to explore the relationships between the role of histone demethylase JMJD2 protein family in bone formation and kidney-invigorating Traditional Chinese Medicine； ②we will then establish the osteoporosis animal models and clinical samples of patients to study the relationship between histone demethylase JMJD2,histone methylation and the development of osteoporosis ,and histone demethylase JMJD2 role in osteoporosis by nourishing kidney method.The completion of this study will reveal molecular mechanisms of histone methylation enzyme and bone formation,so as to osteoporosis.Finally,lead to a novel therapeutic strategy and theory basis for treatment of osteoporosis.