表观基因组学作为在全基因组水平上对表观遗传修饰转变的研究，为人类疾病精准治疗提供了新策略。最新研究证实组蛋白修饰酶可以通过改变组蛋白表观遗传修饰，在骨形成关键基因的表达和功能发挥中起重要作用。我们前期研究发现，组蛋白去甲基化酶JMJD2家族与骨密度及雌激素受体α/β显著正相关，但组蛋白表观遗传修饰在骨质疏松的发生、发展过程中的分布、改变以及补肾法的调控仍需深入探讨。故本项目拟：①采用染色质免疫沉淀-测序（ChIP-seq）等技术手段，借助骨髓间充质干细胞成骨分化模型，骨质疏松动物模型、病人临床样本，研究组蛋白表观遗传修饰与OB、BMSCs骨形成基因、骨转化的关联，及补肾中药和基因组水平组蛋白表观遗传修饰的关系；②采用CRISPR、RNA-seq等技术手段，探明关键组蛋白修饰酶在成骨-破骨耦联中作用机理，进一步阐述与骨质疏松的相关性，为中医药精准防治骨质疏松提供新思路和理论依据。

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell which provides the strategy for precise medicine. Emerging evidence suggests histone modification enzymes play a hugely part in both gene expression and function in bone formation resulted from the change of histone modification. Our previous study indicated that JMJD2 family of histone demethylase is positive related with bone density and estrogen receptor α/β. However, the role of histone epigenetic modification in the development of osteoporosis and its mechanism in “Nourishing Kidney” Chinese formula regulating osteoporosis are still unclear. In this study: ① ChIP-sequencing, RNA-sequencing etc. were used in the models including osteoblasts differentiation of bone mesenchymal stem cells (BMSCs), OVX animals and patients with osteoporosis to find the relationship among the histone epigenetic modification, key genes in bone formation and Chinese herbs; ② CRISP, RNA-sequencing etc were used to explore the mechanism of key histone modification enzymes in the couple of osteoblasts and osteoclasts during the progress of osteoporosis which provide the new way and theoretical evidence for the precise Chinese Medicine in treatment and prophylaxis of osteoporosis.