角膜组织透镜保存再利用的3D参数优化效应的基础研究

飞秒激光小切口透镜取出术是目前重要的角膜屈光手术，本课题组在前期研究中发现飞秒激光三维扫描精确制作的角膜组织透镜在矫正远视、治疗角膜疾病方面具有良好的可行性和潜在应用前景，亟需建立优化的透镜保存方法、明确经保存后的透镜实际屈光力与参数之间的量效规律。本项目拟探讨不同参数及保存时间的角膜组织透镜的光学、组织学特征；建立透镜量效模型—经保存的角膜组织透镜异体移植实际屈光度与透镜参数之间的量效规律；阐明受体角膜透镜术后生物力学对屈光效应的作用规律。拟采用分光光度仪分析光学特征变化；光镜电镜检测角膜胶原纤维变化；Pentacam测量屈光参数；Corvis ST联合Pentacam测量Corvis生物力学指数(CBI)和地形图生物力学指数（TBI）；免疫组化技术检测创伤愈合反应。预期可确立透镜最佳保存期、屈光度量效公式，为进一步建立角膜组织透镜库、角膜组织透镜再利用应用和推广提供理论依据。

Small incision lenticule extraction (SMILE) is an influential refractive surgery technique. Our previous study found that corneal tissue lenticules, precisely extracted by femtosecond laser during SMILE, have broad potential for clinical application, especially in hyperopia correction and treatment of corneal diseases. Therefore, it is important to establish a dose-effect model of preserved corneal lenticules. On this basis, this study aims to determine the correlation between preservation time and different parameters of corneal lenticules (photology and histology). This will establish a dose-effect model which estimates post-allograft refraction, based on the parameters of the preserved corneal lenticule, and demonstrate the effect of biomechanical changes on the corneal refraction of lenticule recipients. Photology is measured by light spectrophotometry. Corneal fibrotic structures are detected via light and electronic microscope. Corneal refractive parameters of recipients’ corneas are measured using Pentacam. Corvis biomechanical index (CBI) and tomographic biomechanical index (TBI) are tested using Corvis ST and Pentacam. Immunohistochemical techniques are applied to monitor the wound healing process. Determining optimal preservation time for corneal tissue lenticules and formulating a dose-effect model of refraction will provide the theoretical basis for future establishment of a corneal lenticule bank, paving the way for growing clinical utilization and popularity of corneal lenticules.