花生钙调素结合蛋白S-腺苷甲硫氨酸合成酶1介导钙离子调控分枝发育分子机理的研究

分枝数是花生重要的植物学性状，与花生产量间呈显著正相关，是促成花生高产的主要因素。目前，人们对植物分枝发育调控分子机制的研究多集中在拟南芥和水稻等模式植物上，对花生分枝的研究还所知甚微。本课题前期从花生中筛选到一个新的钙调素结合蛋白S-腺苷甲硫氨酸合成酶1（SAMS1），过表达该基因的烟草植株分枝数明显增加。以对照花生、过表达AhSAMS1及CaM4和SAMS1双价表达的烟草株系为试材，通过观察花生SAMS1对侧枝形态结构的影响、激素含量的测定、甲基化水平变化及基因调控机理的研究，阐明钙调素结合蛋白S-腺苷甲硫氨酸合成酶1介导钙离子调控分枝发育的分子机制。

The number of branches is an important botanical character of peanut, and it has a positive correlation with the yield of peanut. It is the main factor contributing to the high yield of peanut. At present, there were many studies on the growth and development of branches in the model plants such as Arabidopsis thaliana and rice, but little was known in peanut. A new calmodulin binding protein, S-adenosylmethionine synthase 1 (SAMS1) was identified and the number of tobacco branches that overexpressed this gene increased significantly. Control peanut seedlings, transgene AhSAMS1and CaM4-AhSAMS1 tobacco lines were used as materials. The following trials will be as key ones to be done: the effect of AhSAMS1 on the morphological structure of lateral branch, the determination of hormone content, changes of methylation level and gene regulation analysis, and also to elucidate the mechanism of calcium regulated branching development by peanut calmodulin binding protein S-adenosine methionine synthetase 1.