脂肪组织工程有望成为解决颅颌面软组织缺损或不足的最佳方法，极具潜在临床应用前景。在该研究方向，脂肪基质细胞脂向分化调控机制是关键的研究问题之一。本课题组在前期研究中发现lncRNA-Adi在脂肪基质细胞脂向分化前后有显著差异表达，进一步研究发现lncRNA-Adi与脂向分化关键分子——PPARγ及GATA蛋白具有结合位点，该结果提示lncRNA-Adi极可能通过调控PPARγ信号通路在脂肪基质细胞脂向分化过程中发挥调控作用。因此，本课题拟开展系统研究，明确lncRNA-Adi在脂肪基质细胞脂向分化过程中的表达规律、功能、靶蛋白及相关信号通路，从而了解lncRNA-Adi在这一过程中的具体作用及相关机制。该问题的解决可对明确脂肪基质细胞分化调控机制及改善脂向分化诱导技术提供依据，对了解糖尿病、肥胖症等代谢性疾病的发病机制及探索新型治疗方法也有参考价值。

Adipose tissue engineering could be used to treat cranio-facial soft tissue defects or deficiencies as a best treatment in the future. The regulatory mechanism in ADSCs adipogenic differentiation, especially the epigenetic regulatory mechanism, is the key problem in this research direction. In a previous study, we found a long noncoding RNA which was named with lncRNA-Adi highly expressed differently fore-and aft ADSCs adipogenic differentiation. The following study showed there are some combined motifs between lncRNA-Adi and PPARγ and GATA protein, which are widely accepted as the indispensable key molecules in ADSCs adipogenic differentiation. The above results suggested it very likely that lncRNA-Adi plays an important role by means of controlling PPARγ signal pathway in the regulatory mechanism in ADSCs adipogenic differentiation. So, a systemic study would be performed to explore the expression characteristic, function, target proteins and pathway of lncRNA-Adi in ADSCs adipogenetic differentiation in this project. The results of this study will provide some experimental proofs for understanding the molecular mechanism of ADSCs adipogenetic differentiation and established a new research direction for improve the induction technology of ADSCs adipogenetic differentiation. The results of this study will provide some references to understand the pathogenesis and explore the new therapy method of metabolic diseases, such as diabetes and obesity.