近年来中医药治疗白血病等血液肿瘤疾病的研究多针对免疫调节、增效减毒、诱导凋亡等方面,而中医药干预线粒体氧化呼吸与能量代谢治疗肿瘤的研究尚处空白。线粒体提供细胞90%的能量，在维持细胞功能方面处于重要地位。我们前期研究发现中药复方可影响白血病细胞线粒体凋亡相关因子Bcl-2、caspase-3表达。为进一步研究中医药通过线粒体途径治疗白血病的作用，拟采用KG-1a细胞株在NOD/SCID小鼠平台上建立人急性髓系白血病模型，选用能反映线粒体氧化呼吸与能量代谢功能的线粒体活性氧簇、线粒体膜电位、线粒体呼吸链酶复合体活性、线粒体ATP酶mRNA等指标，探索中医扶正与祛邪疗法干预线粒体氧化呼吸与能量代谢治疗白血病的作用机制，对中医药通过线粒体途径治疗血液肿瘤疾病这一新领域进行探索性研究，为在此理论指导下研究新的抗癌中药提供理论依据。

In recent years, the research priorities of traditional Chinese medicine(TCM) treatment of hematological and neoplastic diseases are immune regulation, synergism and attenuation, apoptosis-inducing and so on. It is still blank that TCM treatment of malignant tumor by intervention of mitochondrial oxidative respiration and energy metabolism. Mitochondria provide 90% energy for cells and it is extremely important to maintain cellular functions. In previous studies, we had discovered Chinese herbal compound could affect expression of two kinds of mitochondria mediated apoptosis related factors(Bcl-2,caspase-3). In order to research TCM treatment of leukemia through mitochondrial pathway, we establish an animal model of acute myelogenous leukemia(AML) by KG-1a cells in NOD/SCID mice. We choose some experimental indexs which could reflect functions of mitochondrial oxidative respiration and energy metabolism, such as mitochondrial reactive oxygen species(ROS), mitochondrial membrane potential, activity of mitochondrial respiratory chain enzyme complexes, expression of mitochondrial ATPase mRNA and so on. According to the experimental data, we hope to clarify the mechanism of TCM Fuzheng and Quxie treatment of leukemia by intervention of mitochondrial oxidative respiration and energy metabolism. We take the study as an exploratory research for the frontier of TCM treatment of hematological malignancies through mitochondrial pathway. Guided by this theory, we could provide the basis for studying new anticancer chinese herbal medicine.